First Report of Fruit Rot of Cherry and Its Control Using FeO Nanoparticles Synthesized in .

Cherry is a fleshy drupe, and it is grown in temperate regions of the world. It is perishable, and several biotic and abiotic factors affect its yield. During April-May 2021, a severe fruit rot of cherry was observed in Swat and adjacent areas. Diseased fruit samples were collected, and the disease-causing pathogen was isolated on PDA. Subsequent morphological, microscopic, and molecular analyses identified the isolated pathogen as . For the control of the fruit rot disease of cherry, iron oxide nanoparticles (FeO NPs) were synthesized in the leaf extract of and characterized. Fourier transform infrared (FTIR) spectroscopy of synthesized FeO NPs showed the presence of capping and stabilizing agents such as alcohols, aldehydes, and halo compounds. X-ray diffraction (XRD) analysis verified the form and size (32 nm) of FeO NPs. Scanning electron microscopy (SEM) revealed the spinal-shaped morphology of synthesized FeO NPs while X-ray diffraction (EDX) analysis displayed the occurrence of main elements in the samples. After successful preparation and characterization of NPs, their antifungal activity against was determined by poison technique. Based on in vitro and in vivo antifungal activity analyses, it was observed that 1.0 mg/mL concentration of FeO can effectively inhibit the growth of fungal mycelia and decrease the incidence of fruit rot of cherry. The results confirmed ecofriendly fungicidal role of FeO and suggested that their large-scale application in the field to replace toxic chemical fungicides.