Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.
Department of Otolaryngology - Head and Neck Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
Methods Mol Biol. 2022;2472:49-56. doi: 10.1007/978-1-0716-2201-8_5.
The Notch pathway regulates many cellular functions in a context-dependent manner. Depending on the cell type, either the activation or inhibition of Notch signaling can influence many processes such as cellular proliferation, specification, differentiation, and survival. The activation of Notch signaling has been shown to have therapeutic advantages in some cancers, thus having a method to identify Notch-activating compounds is needed. In this chapter we outline a method for high-throughput analysis of potential Notch pathway activators in a pancreatic neuroendocrine tumor cell line as an example. We also include the steps for subsequent validation of results and preclinical testing.
Notch 通路以依赖于上下文的方式调节多种细胞功能。根据细胞类型的不同， Notch 信号的激活或抑制均可影响多种过程，如细胞增殖、分化、特化和存活。 Notch 信号的激活已被证明在某些癌症中具有治疗优势，因此需要有一种方法来鉴定 Notch 激活化合物。在本章中，我们概述了一种在胰腺神经内分泌肿瘤细胞系中高通量分析潜在 Notch 通路激活剂的方法，作为示例。我们还包括了后续验证结果和临床前测试的步骤。
