Detection of IncX1 Plasmid-Mediated Gene in an Indian Hospital Sewage Water Using Shotgun Metagenomic Sequencing: A First Report.

Colistin is used against a multitude of multidrug-resistant and extremely drug-resistant Gram-negative bacterial infections. The emergence of colistin resistance is highly concerning as it may lead to the failure of this last-resort antibiotic. Since the identification of first mobile colistin resistance () genes, several variants of genes have been reported, but still there are limited studies detecting genes in hospital sewage water. The prevalence of in the hospital environment is extremely hazardous putting health care workers, patients, and visitors at a higher risk of exposure. It may lead to a multidrug-resistant bacterial infection outbreak. In this study, we report gene in an Indian hospital sewage water using shotgun metagenomics, as a first report. The gene in the metagenome has been explored using RGI, ABRicate, NCBI database, CARD, and Resfinder. This gene harbored by is a plasmid-mediated gene carried by an IncX1 plasmid pSGMCR103. The bioinformatics analysis revealed the genetic environment of gene, which consisted of mobile element protein, ChrB domain protein, putative major facilitator superfamily type transporter, and a hypothetical protein.