Laboratory of Marine Biochemistry, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Nishi-ku Motooka 744, Fukuoka, 819-0395, Japan.
Silkworm Research Group, Division of Silk-Producing Insect Biotechnology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 1-2 Owashi, Tsukuba, Ibaraki, 305-8634, Japan.
Sci Rep. 2022 Jun 8;12(1):9475. doi: 10.1038/s41598-022-13408-6.
Infectious disease is one of the most serious problems in the aquaculture industry for ornamental or edible fish. This study attempted to develop a new device for preventing an aquatic bacterial disease, ulcer disease, caused by Aeromonas salmonicida (As), using "affinity silk". Affinity silk is a silk protein-containing fibroin L-chain (FibL) fused to the single-chain variable fragment (scFv). It can be easily processed into different formats such as fibers, gels, sponges, or films. A transgenic silkworm that could express a cDNA construct containing FibL fused to an scFv derived from a monoclonal antibody (MAb) against As was successfully generated. An enzyme-linked immunosorbent assay was used to detect As by employing 96-well plates coated with scFv-conjugated affinity silk. As could be captured efficiently by glass wool coated with affinity silk in the column. Furthermore, the air-lift water filter equipped with the affinity silk-coated wool could considerably reduce the concentration of As in water and was estimated to have sufficient ability to trap a lethal dose of As. These findings show that the "affinity silk filter" is a potential device for the prophylaxis of aquatic animal diseases.
传染病是水产养殖中观赏鱼或食用鱼面临的最严重问题之一。本研究试图利用“亲和丝”开发一种新的装置来预防由气单胞菌（As）引起的水生细菌性疾病——溃疡病。亲和丝是一种丝蛋白包含的丝素 L 链（FibL）与单链可变片段（scFv）融合的产物。它可以很容易地被加工成不同的形式，如纤维、凝胶、海绵或薄膜。成功地生成了一种能够表达含有 FibL 与抗 As 单克隆抗体（MAb）的 scFv 融合的 cDNA 构建体的转基因蚕。通过在 96 孔板上涂覆 scFv 缀合的亲和丝，酶联免疫吸附测定法（ELISA）可用于检测 As。玻璃纤维棉涂覆的亲和丝可有效地捕获柱中的 As。此外，装有亲和丝涂层羊毛的气升式水过滤器可以显著降低水中 As 的浓度，并且估计具有足够的能力来捕获致死剂量的 As。这些发现表明，“亲和丝过滤器”是一种预防水生动物疾病的潜在装置。
