Department of Stomatology, The Third Affiliated Hospital of Shenzhen University, Shenzhen Luohu Hospital Group Luohu People's Hospital, Shenzhen, Guangdong, China.
J Biomed Mater Res A. 2022 Oct;110(10):1645-1654. doi: 10.1002/jbm.a.37414. Epub 2022 Jun 8.
Soft tissue integration surrounding dental implant is considered as a biological barrier against the oral microorganisms and external stimuli. At present, successful soft tissues integration around implants remains a challenge. The aim of this study was to evaluate the effects of nanostructured zirconia abutment surfaces on fibroblasts viability and Porphyromonas gingivalis adhesion, which are the two main factors influencing the quality of peri-implant soft-tissue seal. Zirconia samples were made from three-dimensional gel deposition approach and divided into three groups: zirconia surfaces, zirconia film coated surfaces (CZr) and polished surfaces (PZr). The Surface properties were characterized by scanning electron microscopy, atomic force microscopy and water contact angle. The behavior of human gingival fibroblasts (HGFs) on samples, including of proliferation, morphology, gene expression and protein expression as well as collagen synthesis were assessed. Additionally, the adhesion of P. gingivalis on samples was analyzed by scanning electron microscopy and live/dead staining. CZr and PZr group significantly enhanced fibroblasts viability such as cell proliferation, cell spreading and extracellular matrix secretion, and impaired P. gingivalis adhesion. Therefore, our findings demonstrated that CZr and PZr surfaces with nano-grains or grinding grooves might be considered as the feasible zirconia abutments substrate for reinforcing HGFs response and minimizing P. gingivalis adhesion, and thereby enhance peri-implant soft-tissue integration.
种植体周围的软组织整合被认为是抵抗口腔微生物和外部刺激的生物屏障。目前，成功的种植体周围软组织整合仍然是一个挑战。本研究旨在评估纳米结构氧化锆基台表面对成纤维细胞活力和牙龈卟啉单胞菌黏附的影响，这是影响种植体周围软组织密封质量的两个主要因素。氧化锆样品采用三维凝胶沉积法制作，并分为三组：氧化锆表面、氧化锆薄膜涂层表面（CCr）和抛光表面（PPr）。通过扫描电子显微镜、原子力显微镜和水接触角对表面特性进行了表征。评估了人牙龈成纤维细胞（HGFs）在样品上的行为，包括增殖、形态、基因表达和蛋白质表达以及胶原蛋白合成。此外，通过扫描电子显微镜和活/死染色分析了 P. gingivalis 在样品上的黏附情况。CCr 和 PPr 组显著提高了成纤维细胞的活力，如细胞增殖、细胞伸展和细胞外基质分泌，并抑制了 P. gingivalis 的黏附。因此，我们的研究结果表明，具有纳米颗粒或研磨槽的 CCr 和 PPr 表面可能被认为是增强 HGFs 反应和最小化 P. gingivalis 黏附的可行氧化锆基台基底，从而增强种植体周围软组织整合。
