Alharthy Saif A, Tabrez Shams, Mirza Ahmed A, Zughaibi Torki A, Firoz Chelapram K, Dutta Mycal
Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
Evid Based Complement Alternat Med. 2022 May 29;2022:7658899. doi: 10.1155/2022/7658899. eCollection 2022.
The diterpenoid, sugiol, has been reported to exert anticancer effects against a number of human cancers. However, the anticancer effects of sugiol have not been evaluated against the human glioma cells. The present study was designed to examine the effects of sugiol on the proliferation of human U87 glioma cells. The results showed that sugiol significantly ( < 0.05) suppressed the viability of the U87 cells in a concentration dependent manner and exhibited an IC value of 15 M. On the other hand, the growth inhibitory effects of sugiol were minimal on the normal human astrocytes. Acridine orange and ethidium bromide staining (AO/EB) staining revealed that sugiol induces apoptosis which was further confirmed by Western blot analysis, wherein upregulation of Bax and downregulation of Bcl-2 were observed in U87 cells. Flow cytometry showed that sugiol causes cell cycle arrest at the / stage. The relative percentage of G1 phase was found to be increased from 26.58% at 0 M to 70.96% at 30 M sugiol. Taken together, the results suggest sugiol inhibits the growth of glioma cells and may prove to be a lead molecule in the management of human glioma.
据报道，二萜类化合物杉醇对多种人类癌症具有抗癌作用。然而，尚未评估杉醇对人胶质瘤细胞的抗癌作用。本研究旨在检测杉醇对人U87胶质瘤细胞增殖的影响。结果表明，杉醇以浓度依赖性方式显著（<0.05）抑制U87细胞的活力，其IC值为15μM。另一方面，杉醇对正常人星形胶质细胞的生长抑制作用极小。吖啶橙和溴化乙锭染色（AO/EB）显示杉醇诱导细胞凋亡，蛋白质免疫印迹分析进一步证实了这一点，其中在U87细胞中观察到Bax上调和Bcl-2下调。流式细胞术显示杉醇使细胞周期停滞在G0/G1期。发现G1期的相对百分比从0μM时的26.58%增加到30μM杉醇时的70.96%。综上所述，结果表明杉醇抑制胶质瘤细胞的生长，可能成为治疗人类胶质瘤的先导分子。
