Tabrez Shams, Khan Azhar U, Hoque Mehboob, Suhail Mohd, Khan Mohammad Imran, Zughaibi Torki A
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Front Chem. 2022 Sep 15;10:970193. doi: 10.3389/fchem.2022.970193. eCollection 2022.
The biogenic approach of synthesizing metal nanoparticles is an exciting and interesting research area with a wide range of applications. The present study reports a simple, convenient, low-cost method for synthesizing magnesium oxide nanoparticles (MgONPs) from pumpkin seed extracts and their anticancer efficacy against ovarian teratocarcinoma cell line (PA-1). The characteristic features of biogenic MgONPs were assessed by UV-visible spectrophotometry (UV-vis), X-ray powder diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The formation of spherical NPs with an average size of 100 nm was observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Moreover, MgONPs exhibit considerable cytotoxicity with an IC dose of 12.5 μg/ml. A dose-dependent rise in the induction of apoptosis, ROS formation, and inhibition in the migration of PA-1 cells was observed up to 15 μg/ml concentration, reflecting their significant anticancer potential against ovarian teratocarcinoma cell line. However, additional work, especially in different and models, is recommended to find out their real potential before this environment-friendly and cost-effective nanoformulation could be exploited for the benefit of humankind.
生物合成金属纳米颗粒的方法是一个令人兴奋且有趣的研究领域，具有广泛的应用。本研究报告了一种从南瓜籽提取物中合成氧化镁纳米颗粒（MgONPs）的简单、便捷、低成本方法及其对卵巢畸胎瘤细胞系（PA-1）的抗癌功效。通过紫外可见分光光度法（UV-vis）、X射线粉末衍射（XRD）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）对生物合成的MgONPs的特征进行了评估。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察到形成了平均尺寸为100 nm的球形纳米颗粒。此外，MgONPs在IC剂量为12.5 μg/ml时表现出相当大的细胞毒性。在浓度高达15 μg/ml时，观察到PA-1细胞凋亡诱导、ROS形成和迁移抑制呈剂量依赖性增加，这反映了它们对卵巢畸胎瘤细胞系具有显著的抗癌潜力。然而，建议开展更多工作，尤其是在不同的[此处原文缺失相关内容]和模型中，以便在这种环境友好且经济高效的纳米制剂能够造福人类之前，了解它们的实际潜力。
