Miao Yulu, Wang Bin, Hu Jing, Zhang Hanyu, Li Xiaojin, Huang Yingying, Zhuang Pengwei, Zhang Yanjun
Chinese Materia Medica College, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
College of Pharmacy, Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine, Hefei, China.
Front Pharmacol. 2022 May 23;13:888684. doi: 10.3389/fphar.2022.888684. eCollection 2022.
Lung infection is a common complication induced by stroke and seriously affects the prognosis and life quality of patients. However, effective therapeutic strategies are still lacking. In the present study, the herb formula GCis was confirmed to prevent pulmonary infection induced by intracerebral hemorrhage (ICH). The animal model of lung infection induced by ICH, GCis (Ginseng Radix et Rhizoma, Aconiti Lateralis Radix Praeparata, and Cistanches Herba) was orally administrated every day for 7 days. Lung microbial biomass and pathological results showed that the GCis formula pretreatment significantly reduced lung bacterial biomass and alleviated pathological abnormalities. These results indicated that the GCis formula has a clear pharmacological effect on preventing lung infection induced by ICH. Immunosuppression induced by ICH seemed to be the main mechanism of lung infection. Our results showed that the spleen and thymus indexes, WBC, and LY% contents were significantly increased in the GCis formula group. Moreover, bone marrow cells were further analyzed by transcriptome sequencing, and GO and KEGG enrichment analysis results showed that immune function was the main pathway enriched by differential genes after GCis formula intervention. More importantly, our results showed that GCis pretreatment had no significant effect on the mRNA expression of IL-1β, IL-6, and TNF-α in the brain. These results indicated that the GCis formula could enhance immunity after ICH. The intestinal barrier function was further investigated in the present study, considering the origin of the source of infection. Our results showed that the mRNA expressions of intestinal ZO-1, SIgA, and MUC2 were significantly increased, villi structure was intact, inflammatory cell infiltration was reduced, and goblet cell number was increased after GCis formula treatment. These results suggest that the GCis formula can enhance the intestinal mucosal immune barrier. This study provides a herb formula (GCis) that could enhance peripheral immunity and intestinal mucosal immune barrier to prevent pulmonary infection induced by ICH. It would be beneficial in the prevention of severe clinical infections.
肺部感染是中风引发的常见并发症，严重影响患者的预后和生活质量。然而，目前仍缺乏有效的治疗策略。在本研究中，中药复方GCis（人参、制附子、肉苁蓉）被证实可预防脑出血（ICH）诱发的肺部感染。采用ICH诱发肺部感染的动物模型，每天口服GCis 7天。肺部微生物量及病理结果显示，GCis复方预处理显著降低了肺部细菌量，并减轻了病理异常。这些结果表明，GCis复方对预防ICH诱发的肺部感染具有明确的药理作用。ICH诱导的免疫抑制似乎是肺部感染的主要机制。我们的结果显示，GCis复方组的脾脏和胸腺指数、白细胞及淋巴细胞百分比含量显著增加。此外，通过转录组测序对骨髓细胞进行进一步分析，GO和KEGG富集分析结果显示，免疫功能是GCis复方干预后差异基因富集的主要通路。更重要的是，我们的结果显示，GCis预处理对大脑中白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α的mRNA表达无显著影响。这些结果表明，GCis复方可在ICH后增强免疫力。考虑到感染源的起源，本研究进一步探讨了肠道屏障功能。我们的结果显示，GCis复方治疗后，肠道紧密连接蛋白-1、分泌型免疫球蛋白A和黏蛋白2的mRNA表达显著增加，绒毛结构完整，炎性细胞浸润减少，杯状细胞数量增加。这些结果表明，GCis复方可增强肠道黏膜免疫屏障。本研究提供了一种可增强外周免疫力和肠道黏膜免疫屏障以预防ICH诱发肺部感染的中药复方（GCis）。这将有助于预防严重的临床感染。
