Mohammed Abde Aliy, Chibssa Tesfaye Rufael, Terfa Waktole, Aklilu Fasil, Damena Delesa, Belayneh Redeat, Kidane Menbere
National Animal Health Diagnostic and Investigation Center (NAHDIC), P.O. Box 04, Sebeta, Ethiopia.
Ambo University, School of Veterinary Sciences, P.O. Box 19, Ambo, Ethiopia.
Adv Virol. 2022 May 29;2022:5329898. doi: 10.1155/2022/5329898. eCollection 2022.
Peste des petits ruminants (PPR) is one of the most important transboundary diseases of small ruminants. In this study, nasal and oral swabs ( = 24) were collected from sheep ( = 7) and goats ( = 17) with clinical signs in southern Ethiopia in March 2020. PPR virus was isolated on Vero dog cells expressing the signaling lymphocyte activation molecule (VDS) and screened using RT-qPCR. Positive samples were confirmed by conventional RT-PCR followed by sequencing of a partial nucleoprotein (N) gene segment. Results revealed that 54% ( = 13/24) of the tested samples were PPRV-positive Phylogenetic analysis revealed that the viruses belonged to lineage IV and lineage II. The lineage IV viruses were similar, although not identical, to other lineage IV viruses previously reported in Ethiopia and other East African countries while the lineage II viruses have been reported for the first time in Ethiopia showed a high nucleotide identity (99.06%) with the vaccine (Nigeria 75/1) that is currently used in Ethiopia for the prevention of PPR. Further investigations are therefore recommended in order to fully understand the true nature of the lineage II PPRVs in Ethiopia.
小反刍兽疫（PPR）是小反刍动物最重要的跨境疾病之一。在本研究中，2020年3月从埃塞俄比亚南部出现临床症状的绵羊（n = 7）和山羊（n = 17）采集了鼻拭子和口拭子（n = 24）。在表达信号淋巴细胞激活分子的Vero犬细胞（VDS）上分离小反刍兽疫病毒，并使用RT-qPCR进行筛选。通过常规RT-PCR对阳性样本进行确认，随后对部分核蛋白（N）基因片段进行测序。结果显示，54%（n = 13/24）的检测样本为小反刍兽疫病毒阳性。系统发育分析表明，这些病毒属于IV系和II系。IV系病毒与先前在埃塞俄比亚和其他东非国家报道的其他IV系病毒相似，但并不完全相同，而II系病毒是在埃塞俄比亚首次报道，与目前在埃塞俄比亚用于预防小反刍兽疫的疫苗（尼日利亚75/1）具有较高的核苷酸同一性（99.06%）。因此，建议进一步开展调查，以全面了解埃塞俄比亚II系小反刍兽疫病毒的真实性质。
