Department of Microbial Cellular and Molecular Biology, Addis Ababa University, P.O. Box: 1176, Ethiopia; African Union- Pan African Veterinary Vaccine Centre (AU-PANVAC), P. O. Box 1746, Debre Zeit, Ethiopia.
Animal Production and Health Laboratory, Animal Production and Health Section, Joint FAO/IAEA Division, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria.
Vet Microbiol. 2019 Aug;235:229-233. doi: 10.1016/j.vetmic.2019.07.006. Epub 2019 Jul 11.
Peste des Petits ruminants (PPR) is a devastating disease of small ruminants with high morbidity and mortality rates among susceptible animals. The disease is endemic in much of Africa, the Middle East and Asia and constitutes one of the major hurdles to the improvement of small-ruminant production in these countries. The causal agent of PPR, the Small Ruminant Morbillivirus (SRMV), previously known as PPR virus (PPRV) belongs to the genus Morbillivirus within the family Paramyxoviridae. SRMV can be categorized into four genetically distinct lineages (I to IV). Suspicion of PPR was first reported in Ethiopia in 1977 and since then genetic characterization of circulating viruses has identified lineages III and IV in the country. This study was undertaken to provide an update on the molecular epidemiology of PPR in Ethiopia by analysing animal tissue samples collected between 2011 and 2017. PPR positive samples were identified in four regions of the country. Sequence and phylogenetic analysis of fourteen RT-PCR positive amplicons revealed that all of the SRMV in the samples from 2010 to 2017 belong to sub-clade II of clade I of lineage IV. No lineage III viruses were identified.
小反刍兽疫（PPR）是一种对小反刍动物具有高发病率和死亡率的毁灭性疾病。该疾病在非洲大部分地区、中东和亚洲流行，是这些国家提高小反刍动物生产的主要障碍之一。小反刍兽疫的病原体是小反刍兽麻疹病毒（SRMV），以前称为小反刍动物病毒（PPRV），属于副粘病毒科麻疹病毒属。SRMV 可分为四个遗传上不同的谱系（I 至 IV）。1977 年，埃塞俄比亚首次报告了对 PPR 的怀疑，自那时以来，对循环病毒的遗传特征分析表明，该国存在谱系 III 和 IV。本研究通过分析 2011 年至 2017 年收集的动物组织样本，对埃塞俄比亚的 PPR 分子流行病学进行了更新。在该国的四个地区发现了 PPR 阳性样本。对 14 个 RT-PCR 阳性扩增子的序列和系统发育分析表明，2010 年至 2017 年所有样本中的 SRMV 均属于 IV 谱系 I 分支的亚分支 II。未鉴定出线粒体 III 病毒。
