Department of Biological Sciences, University of Toledo, Toledo, OH 43606, USA.
Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
STAR Protoc. 2022 Jun 4;3(2):101437. doi: 10.1016/j.xpro.2022.101437. eCollection 2022 Jun 17.
Ruffles are actin-rich membrane protrusions implicated in actin reorganization and initiation of cell motility. Here, we describe methods for measuring and analyzing ruffle dynamics in live cells and average ruffle area per cell in fixed samples. The specific steps described are for the analysis of A549 lung adenocarcinoma cells, but the protocol can be applied to other cell types. The protocol has applications for dissecting the signaling events linked to ruffling. For complete details on the use and execution of this protocol, please refer to Cooke et al. (2021).
褶边是富含肌动蛋白的膜突，参与肌动蛋白重组和细胞运动的起始。在这里，我们描述了测量和分析活细胞中褶边动力学以及固定样本中每个细胞平均褶边面积的方法。所描述的具体步骤是针对肺腺癌细胞 A549 的分析，但该方案可应用于其他细胞类型。该方案可用于剖析与褶边相关的信号事件。有关此方案的使用和执行的完整详细信息，请参阅 Cooke 等人。（2021 年）。
