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在459个样本中,于花期诱导过程中收集的转录组(RNA测序)数据集。 (原英文表述似乎不太完整准确,按字面意思大致如此翻译)

The transcriptomic (RNA-Sequencing) datasets collected in the course of floral induction in 459.

作者信息

Gutierrez-Larruscain David, Krüger Manuela, Abeyawardana Oushadee A J, Belz Claudia, Dobrev Petre I, Vaňková Radomíra, Eliášová Kateřina, Vondráková Zuzana, Juříček Miloslav, Štorchová Helena

机构信息

Institute of Experimental Botany, Czech Academy of Sciences, Rozvojová 263, 16502 Prague, Czech Republic.

出版信息

Data Brief. 2022 May 29;43:108333. doi: 10.1016/j.dib.2022.108333. eCollection 2022 Aug.

Abstract

The transition from vegetative growth to reproduction is the essential commitment in plant life. It is triggered by environmental cues (day length, temperature, nutrients) and regulated by the very complex signaling gene network and by phytohormones. The control of flowering is well understood in and in some crops, much less is known about the other angiosperms. We performed the detailed transcriptomic survey of the course of floral induction in seedlings of accession 459, a close relative of the important crop . It flowers earlier under short days (6 hours light) than under long days (18 hours light). Plants were sampled at the age 14, 18, 21 and 24 days in the morning and afternoon, both at long and short day, for RNA-Sequencing, and also for phytohormone analyses. We employed Illumina NovaSeq6000 platform to generate raw reads, which were cleaned and mapped against the constructed transcriptome of . The global gene expression levels between long and short days were pairwise compared at each time points. We identified differentially expressed genes associated with floral induction in 459. Particular attention was paid to the genes responsible for phytohormone metabolism and signaling. The datasets produced by this project contributed to better understanding of the regulation of growth and development in the genus .

摘要

从营养生长向生殖生长的转变是植物生命中的关键过程。它由环境信号(日照长度、温度、养分)触发,并受非常复杂的信号基因网络和植物激素调控。在[具体植物名称1]以及一些作物中,开花的调控已得到充分了解,但对于其他被子植物的了解则少得多。我们对重要作物[具体植物名称2]的近缘种459号种质的幼苗进行了详细的转录组学研究,以探究其成花诱导过程。该种质在短日照(6小时光照)下比在长日照(18小时光照)下开花更早。在长日照和短日照条件下,分别于植株14、18、21和24日龄的上午和下午进行采样,用于RNA测序以及植物激素分析。我们使用Illumina NovaSeq6000平台生成原始读数,对其进行清理后与构建的[具体植物名称2]转录组进行比对。在每个时间点对长日照和短日照条件下的全局基因表达水平进行两两比较。我们鉴定出了与459号种质成花诱导相关的差异表达基因。特别关注了负责植物激素代谢和信号传导的基因。该项目产生的数据集有助于更好地理解[具体植物名称2]属植物的生长和发育调控。

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