埃塞俄比亚亚的斯亚贝巴一个表面健康人群中CD4 T淋巴细胞绝对值和百分比的参考区间。

Reference Intervals for Absolute and Percentage CD4 T Lymphocytes among an Apparently Healthy Population in Addis Ababa, Ethiopia.

作者信息

Chalchisa Dinkenesh, Belay Yohannes, Befekadu Endalkachew, Kassaw Melkitu, G/Egzeabher Letebrhan, Gebremicael Gebremedhin, Lengiso Boki, Chala Dawit, Sahlemariam Zewdineh, Kebede Estifanos, Abate Ebba, Tsegaye Aster

机构信息

National HIV Reference Laboratory, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.

Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.

出版信息

Int J Gen Med. 2022 Jun 1;15:5361-5367. doi: 10.2147/IJGM.S357237. eCollection 2022.

DOI:10.2147/IJGM.S357237

PMID:35677805

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9167834/

Abstract

BACKGROUND

Reference intervals for clinical laboratory parameters differ based on several factors, including age, sex, genetic variation, and geographic location. This variation influences clinical decisions and treatment monitoring. Currently, Ethiopia has used adopted reference intervals from manufacturer values derived from non-Africans. Therefore, the aim this study was to determine reference intervals for absolute and percentage CD4 T cells for an apparently healthy population in Addis Ababa, Ethiopia.

METHODS

A community-based cross-sectional study was conducted on 361 apparently healthy people in four subcities in Addis Ababa from January to June 2019. Sociodemographic and clinical data were collected using a structured questionnaire after informed consent had been obtained. Blood samples were collected and CD4 T-lymphocyte enumeration performed using a BD FACSPresto near-patient CD4 counter. Data were entered and analyzed using SPSS 20. Reference intervals were determined by a nonparametric test estimating percentiles 2.5 (lower limit) and 97.5 (upper limit) with 95% CIs. <0.05 was considered statistically significant.

RESULTS

A total of 337 (183 female and 154 male) healthy participants of median age 28 (IQR 17-35) years were included in the final analysis. Medians of absolute and percentage CD4 T-cell counts (932.0 and 42.9, respectively) of female participants were significantly higher than male participants (802.5 and 38.7, respectively; <0.05). Reference intervals for absolute CD4 T-cell count and percentages in males were 483.8-1,310 cells/µL and 18.1-57.3 and in females 447.8-1,479.8 cells/µL and 25.6-58.9, respectively.

CONCLUSION

The CD4 T-count reference intervals established in this study showed some inconsistency from the manufacturer's provided values and other studies and also revealed sex differences, necessitating sex-specific locally established reference intervals.

摘要

背景

临床实验室参数的参考区间因多种因素而异，包括年龄、性别、基因变异和地理位置。这种差异会影响临床决策和治疗监测。目前，埃塞俄比亚采用的是源自非非洲人的制造商值的参考区间。因此，本研究的目的是确定埃塞俄比亚亚的斯亚贝巴明显健康人群的绝对和百分比CD4 T细胞参考区间。

方法

2019年1月至6月，在亚的斯亚贝巴的四个分区对361名明显健康的人进行了一项基于社区的横断面研究。在获得知情同意后，使用结构化问卷收集社会人口学和临床数据。采集血样并使用BD FACSPresto床边CD4计数仪进行CD4 T淋巴细胞计数。数据录入并使用SPSS 20进行分析。通过非参数检验确定参考区间，估计第2.5百分位数（下限）和第97.5百分位数（上限）以及95%置信区间。P<0.05被认为具有统计学意义。

结果

最终分析纳入了337名健康参与者（183名女性和154名男性），中位年龄为28岁（四分位间距17 - 35岁）。女性参与者的绝对和百分比CD4 T细胞计数中位数（分别为932.0和42.9）显著高于男性参与者（分别为802.5和38.7；P<0.05）。男性绝对CD4 T细胞计数和百分比的参考区间分别为483.8 - 1310个细胞/微升和18.1 - 57.3，女性分别为447.8 - 1479.8个细胞/微升和25.6 - 58.9。