Bikila Demiraw, Lejisa Tadesse, Tolcha Yosef, Bashea Chala, Meles Mehari, Getahun Tigist, Ashebir Genet, Habtu Wossene, Challa Feyissa, Mohammed Ousman, Kassaw Melkitu, Kebede Adisu, G/Egzeabher Letebrhan, Befekadu Endalkachew, Wolde Mistire, Tsegaye Aster
Department of National Clinical Chemistry Reference Laboratory, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Department of Medical Laboratory Sciences, Wollo University, Dessie, Ethiopia.
Int J Gen Med. 2022 Aug 23;15:6701-6711. doi: 10.2147/IJGM.S376450. eCollection 2022.
Even though several factors affect reference intervals (RIs), company-derived values are currently in use in many laboratories worldwide. However, few or no data are available regarding serum proteins RIs, especially in resource-limited countries such as Ethiopia.
To establish RIs for serum protein electrophoresis of apparently healthy adults in Addis Ababa, Ethiopia.
A cross-sectional study was conducted on a total of 297 apparently healthy adults from April to October 2019 in four selected sub-cities (Akaki, Kirkos, Arada, and Yeka) of Addis Ababa, Ethiopia. Laboratory analysis of collected samples was performed using the Capillarys 2 Flex Piercing analyzer, while statistical analysis was conducted using SPSS version 23 and Med-Calc software. The Mann-Whitney test was used to check partitions. A non-parametric method of reference range establishment was performed as per CLSI guideline EP28A3C.
The established RIs were: albumin 53.83-64.59%, 52.24-63.55%; alpha-1 globulin 3.04-5.40%, 3.44-5.60%; alpha-2 globulin 8.0-12.67%, 8.44-12.87%; and beta-1 globulin 5.01-7.38%, 5.14-7.86%. Moreover, the albumin to globulin ratios were 1.16-1.8 and 1.09-1.74 for males and females, respectively. The combined RIs for beta-2 globulin and gamma globulin were 2.54-4.90% and 12.40-21.66%, respectively.
The established reference interval for serum protein fractions revealed gender-specific differences, except for beta-2 globulin and gamma globulin.
尽管有几个因素会影响参考区间（RI），但目前全球许多实验室仍在使用公司提供的数值。然而，关于血清蛋白参考区间的数据很少，甚至没有，尤其是在像埃塞俄比亚这样资源有限的国家。
建立埃塞俄比亚亚的斯亚贝巴明显健康成年人血清蛋白电泳的参考区间。
2019年4月至10月，在埃塞俄比亚亚的斯亚贝巴四个选定的子城市（阿卡基、基尔科斯、阿拉达和耶卡）对总共297名明显健康的成年人进行了横断面研究。使用Capillarys 2 Flex Piercing分析仪对采集的样本进行实验室分析，同时使用SPSS 23版和Med-Calc软件进行统计分析。采用曼-惠特尼检验来检查分组情况。根据CLSI指南EP28A3C采用非参数方法建立参考范围。
建立的参考区间为：白蛋白53.83 - 64.59%，52.24 - 63.55%；α1球蛋白3.04 - 5.40%，3.44 - 5.60%；α2球蛋白8.0 - 12.67%，8.44 - 12.87%；β1球蛋白5.01 - 7.38%，5.14 - 7.86%。此外，男性和女性的白蛋白与球蛋白比值分别为1.16 - 1.8和1.09 - 1.74。β2球蛋白和γ球蛋白的合并参考区间分别为2.54 - 4.90%和12.40 - 21.66%。
除β2球蛋白和γ球蛋白外，建立的血清蛋白组分参考区间显示出性别差异。
