Department of Medical Laboratory, College of Medicine and Health Science, Hawassa University, Comprehensive Specialized Hospital, Hawassa, Sidama Region, Ethiopia.
School of Medical Laboratory Sciences, Institute of Health Faculty of Health, Jimma University, Jimma, Oromiya Region, Ethiopia.
PLoS One. 2021 Apr 8;16(4):e0249185. doi: 10.1371/journal.pone.0249185. eCollection 2021.
Pregnancy is a state characterized by physiological, hematological, and immunological changes. However, the reference intervals (RI) being used in clinical practice in Ethiopia are derived from non-local general populations. Therefore; this study was aimed to determine the reference interval of hematological and immunological profiles among healthy pregnant mothers attending Hawassa University Hospital.
A cross-sectional study in a total of 360 healthy pregnant women was enrolled from January to April 2019, at Hawassa University hospital. Sociodemographic and obstetric data were collected using a structured questionnaire. Blood samples collected from each participant were used to define the hematological parameters. The median and 95% intervals were calculated for the immunological and hematological profiles. P-value 0.05 was considered statistically significant.
A total of 360 healthy pregnant women were enrolled in this study. The age range of the participants was 18-45 years. 342(95%) were married and 270 (75%) of the participants were multigravida. The overall median CD4+ T-cell and total WBC counts (cells/mm3) were 602 and 7.58 respectively. The overall median value for lymphocytes, neutrophils, monocytes, eosinophils, and basophil count was (cells/mm3) was 2.21, 6.74, .63, .53, and 0.09 respectively. Whereas the median RBC and platelet count was 4.48×106/μLand 212×106/μL. The median value of hematological profiles in the first, second, and third trimesters was TWBC (103/μL) (7.90, 8.30, 8.65), RBC (106/μL) (4.5, 4.6, 4.62), and PLT (103/μL) (210, 209,161) respectively. The CD4 T cell count median value was (600, 598, and 591) in the first, second, and third trimesters. Significant changes were observed in hematological and immunological parameters between trimesters (P < 0.05).
Significant changes were observed in hematological and immunological parameters between trimesters (P < 0.05). Considerable differences were also seen between the values in this study and other studies from Ethiopia and other countries, indicated the need for the development of local reference intervals for pregnant women.
妊娠是一种以生理、血液和免疫变化为特征的状态。然而，埃塞俄比亚临床实践中使用的参考区间是从非本地一般人群中得出的。因此；本研究旨在确定在 Hawassa 大学医院就诊的健康孕妇的血液学和免疫学特征参考区间。
这是一项 2019 年 1 月至 4 月期间在 Hawassa 大学医院共纳入 360 名健康孕妇的横断面研究。使用结构化问卷收集社会人口学和产科数据。从每位参与者采集的血液样本用于确定血液学参数。计算了免疫学和血液学特征的中位数和 95%区间。P 值<0.05 被认为具有统计学意义。
本研究共纳入 360 名健康孕妇。参与者的年龄范围为 18-45 岁。342 名（95%）已婚，270 名（75%）为多产妇。总的 CD4+T 细胞和总白细胞计数（细胞/mm3）分别为 602 和 7.58。总的淋巴细胞、中性粒细胞、单核细胞、嗜酸性粒细胞和嗜碱性粒细胞计数的中位数（细胞/mm3）分别为 2.21、6.74、.63、.53 和 0.09。而红细胞和血小板计数的中位数分别为 4.48×106/μL 和 212×106/μL。第一、二、三孕期的血液学参数中位数分别为 TWBC（103/μL）（7.90、8.30、8.65）、RBC（106/μL）（4.5、4.6、4.62）和 PLT（103/μL）（210、209、161）。CD4 T 细胞计数中位数分别为第一、二、三孕期的 600、598 和 591。各孕期之间的血液学和免疫学参数有显著变化（P<0.05）。
各孕期之间的血液学和免疫学参数有显著变化（P<0.05）。本研究中的值与埃塞俄比亚和其他国家的其他研究值之间存在相当大的差异，表明需要为孕妇制定本地参考区间。
