Guo Chen, Jiang Xinbang, Guo Xiaofang, Liu Zhuang, Wang Biao, Du Yunzheng, Tian Ziying, Wang Zimeng, Ou Lailiang
Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Science, Nankai University, Tianjin 300071, China.
Regen Biomater. 2024 Apr 29;11:rbae045. doi: 10.1093/rb/rbae045. eCollection 2024.
Selective removal of ultra-high low-density lipoprotein (LDL) from the blood of hyperlipemia patients using hemoperfusion is considered an efficient method to prevent the deterioration of atherosclerotic cardiovascular disease. Based on the exceptional structure-function properties of multistimulus-responsive materials, we developed a magnetic photorenewable nanoadsorbent (FeO@SiO@Azo-COOH) with outstanding selectivity and regenerative characteristics, featuring functionalized azobenzene as the ligand. The dual-stimulus response endowed FeO@SiO@Azo-COOH with rapid separation and photoregenerative properties. The adsorbent demonstrated excellent removal efficiency of LDL with an adsorption capacity of 15.06 mg/g, and highly repetitive adsorption performance (≥5 cycles) under irradiation. FeO@SiO@Azo-COOH also exhibited remarkable adsorption properties and selectivity in human serum, with adsorption capacities of 10.93, 21.26 and 9.80 mg/g for LDL, total cholesterol and triglycerides and only 0.77 mg/g for high-density lipoprotein (HDL), resulting in a 93% selective adsorption difference (LDL/HDL). Complete green regeneration of the nanoadsorbent was achieved through a simple regeneration process, maintaining a recovery rate of 99.4% after five regeneration experiments. By combining dynamic perfusion experiment with micromagnetic microfluidics, the LDL content decreased by 16.6%. Due to its superior adsorption capacity and regenerative properties, the dual stimulus-responsive nanosorbent is considered a potential hemoperfusion adsorbent.
使用血液灌流从高脂血症患者血液中选择性去除超高密度脂蛋白（LDL）被认为是预防动脉粥样硬化性心血管疾病恶化的有效方法。基于多刺激响应材料卓越的结构功能特性，我们开发了一种具有出色选择性和再生特性的磁性光可再生纳米吸附剂（FeO@SiO@Azo-COOH），其以功能化偶氮苯作为配体。双刺激响应赋予FeO@SiO@Azo-COOH快速分离和光再生性能。该吸附剂对LDL的去除效率极佳，吸附容量为15.06 mg/g，在光照下具有高度重复的吸附性能（≥5个循环）。FeO@SiO@Azo-COOH在人血清中也表现出显著的吸附性能和选择性，对LDL、总胆固醇和甘油三酯的吸附容量分别为10.93、21.26和9.80 mg/g，而对高密度脂蛋白（HDL）的吸附容量仅为0.77 mg/g，导致选择性吸附差异为93%（LDL/HDL）。通过简单的再生过程实现了纳米吸附剂的完全绿色再生，经过五次再生实验后回收率保持在99.4%。通过将动态灌注实验与微磁微流体相结合，LDL含量降低了16.6%。由于其优异的吸附容量和再生性能，这种双刺激响应纳米吸附剂被认为是一种潜在的血液灌流吸附剂。
