Ward B G, Wallace K, Shepherd J H, Balkwill F R
Cancer Res. 1987 May 15;47(10):2662-7.
Using continuous human ovarian cancer cell lines, i.p. xenografts were successfully established in nude mice from four of four attempts. When primary tumor material was used, xenografts grew in 8 of 10 attempts. From these eight, three passageable xenograft cell lines have been established. To our knowledge, this is the first report published of such xenografts. I.p. xenografts closely mimic the clinical behavior of human ovarian cancer, and those developed from primary tumor material maintain close morphological similarity to the parent primary tumor. When expression of placental alkaline phosphatase and the tumor associated antigens defined by the monoclonal antibodies HMFG1, HMFG2, AUA1, and F36/22 by these models was determined, those i.p. xenografts derived from primary tumor material exactly matched the original tumor, while none of the xenografts derived from the cell lines expressed these antigens. These models will be useful for investigating the biology and treatment of ovarian cancer.
利用人卵巢癌细胞系，在4次尝试中均成功地在裸鼠体内建立了腹腔异种移植瘤。当使用原发性肿瘤材料时，10次尝试中有8次异种移植瘤生长。从这8个中，已经建立了3个可传代的异种移植细胞系。据我们所知，这是首次发表的关于此类异种移植瘤的报告。腹腔异种移植瘤紧密模拟人卵巢癌的临床行为，而从原发性肿瘤材料发展而来的异种移植瘤与亲本原发性肿瘤在形态上保持密切相似性。当通过这些模型测定胎盘碱性磷酸酶以及单克隆抗体HMFG1、HMFG2、AUA1和F36/22所定义的肿瘤相关抗原的表达时，那些源自原发性肿瘤材料的腹腔异种移植瘤与原始肿瘤完全匹配，而源自细胞系的异种移植瘤均未表达这些抗原。这些模型将有助于研究卵巢癌的生物学特性和治疗方法。
