School of Life Sciences, Zhengzhou University, Zhengzhou, PR China.
Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, PR China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2022 Aug;39(8):1390-1400. doi: 10.1080/19440049.2022.2081366. Epub 2022 Jun 9.
Based on a highly sensitive and specific monoclonal antibody (mAb) against virginiamycin M1 (VIR M1), a quantum dots-based fluorescent immunochromatographic assay (QDs-ICA) for quick and sensitive analysis of VIR M1 was established for the first time. The mAb showed a half-maximal inhibitory concentration (IC) of 0.5 ng/mL and cross-reactivity (CR) values below 0.1% for other three analogues when used in enzyme-linked immunosorbent assay (ELISA). The mAb was conjugated to ZnCdSe/ZnS (core/shell) QDs with maximum emission wavelength of 610 nm (orange-red) which was selected as fluorescent probe to increase QDs-ICA sensitivity. The cut-off value of QDs-ICA was 12.5 ng/mL. QDs-ICA showed a linear range from 0.7 to 14.5 ng/mL with a limit of quantification of 0.7 ng/mL. Compared with existing methods for the analysis of VIR M1, the QDs-ICA exhibited higher sensitivity. For analysis of VIR M1 concentrations spiked into swine feed, muscle and liver samples, recovery rates ranged from 94.0% to 111.6% with the highest coefficient of variation (CV) of 6.7% for intra-assay, and for inter-assay ranged from 94.7% to 107.6% with the highest CV of 9.4%. In conclusion, the QDs-ICA could be a potential method for analyzing VIR M1 in animal feed and animal-derived food.
基于针对维吉霉素 M1（VIR M1）的高度敏感和特异的单克隆抗体（mAb），首次建立了用于快速灵敏分析 VIR M1 的量子点荧光免疫层析测定法（QDs-ICA）。该 mAb 在酶联免疫吸附测定（ELISA）中对其他三种类似物的半抑制浓度（IC）为 0.5ng/mL，交叉反应（CR）值低于 0.1%。该 mAb 与最大发射波长为 610nm（橙红色）的 ZnCdSe/ZnS（核/壳）量子点偶联，作为荧光探针以提高 QDs-ICA 的灵敏度。QDs-ICA 的截止值为 12.5ng/mL。QDs-ICA 的线性范围为 0.7 至 14.5ng/mL，定量下限为 0.7ng/mL。与现有的 VIR M1 分析方法相比，QDs-ICA 具有更高的灵敏度。对于添加到猪饲料、肌肉和肝脏样品中的 VIR M1 浓度的分析，回收率在 94.0%至 111.6%之间，内部测定的最高变异系数（CV）为 6.7%，而对于间测定，回收率在 94.7%至 107.6%之间，最高 CV 为 9.4%。总之，QDs-ICA 可能是分析动物饲料和动物源性食品中 VIR M1 的一种潜在方法。
