Anhui Province Key Laboratory of Crop Integrated Pest Management, Anhui Province Engineering Laboratory for Green Pesticide Development and Application, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Ecotoxicol Environ Saf. 2022 Aug;241:113738. doi: 10.1016/j.ecoenv.2022.113738. Epub 2022 Jun 6.
The involvement of carboxylesterases (CarEs) in resistance to chlorpyrifos has been confirmed by the synergism analysis in Nilaparvata lugens. However, the function of specific CarE gene in chlorpyrifos resistance and the transcriptional regulatory mechanism are obscure. Herein, the expression patterns of 29 CarE genes in the susceptible and chlorpyrifos-resistant strains were analyzed. Among them, CarE3, CarE17 and CarE19 were overexpressed in the resistant strain, and knockdown of either CarE gene by RNA interference significantly increased the susceptibility to chlorpyrifos. Remarkably, knockdown of CarE17 reduced the enzymatic activity of CarE by 88.63 % and showed a much greater effect on increasing chlorpyrifos toxicity than silencing other two CarE genes. Overexpression of CarE17 in Drosophila melanogaster decreased the toxicity of chlorpyrifos to transgenic fruit flies. Furthermore, the region between - 205 to + 256 of CarE17 promoter sequence showed the highest promoter activity, and 16 transcription factors (TFs) were predicted from this region. Among these TFs, Lim1β and C15 were overexpressed in the resistant strain. Knockdown of either TF resulted in reduced CarE17 expression and a decrease in resistance of N. lugens to chlorpyrifos. These results indicate that the constitutive overexpression of Lim1β and C15 induces CarE17 expression thus conferring chlorpyrifos resistance in N. lugens.
羧酸酯酶（CarE）参与了毒死蜱抗性，这已通过褐飞虱的增效作用分析得到证实。然而，特定的 CarE 基因在毒死蜱抗性中的功能和转录调控机制尚不清楚。在此，分析了敏感和抗毒死蜱品系中 29 个 CarE 基因的表达模式。其中，CarE3、CarE17 和 CarE19 在抗性品系中过表达，通过 RNA 干扰敲低任一 CarE 基因均显著增加了对毒死蜱的敏感性。值得注意的是，CarE17 的敲低使 CarE 的酶活性降低了 88.63%，对增加毒死蜱毒性的影响比沉默其他两个 CarE 基因大得多。在黑腹果蝇中过表达 CarE17 降低了转基因果蝇对毒死蜱的毒性。此外，CarE17 启动子序列的-205 至+256 区域显示出最高的启动子活性，并且从该区域预测了 16 个转录因子（TFs）。在这些 TFs 中，Lim1β 和 C15 在抗性品系中过表达。敲低任一 TF 均导致 CarE17 表达减少，褐飞虱对毒死蜱的抗性降低。这些结果表明，Lim1β 和 C15 的组成型过表达诱导了 CarE17 的表达，从而赋予褐飞虱对毒死蜱的抗性。
