Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China.
Biosens Bioelectron. 2016 May 15;79:341-6. doi: 10.1016/j.bios.2015.12.040. Epub 2015 Dec 17.
A novel fluorescence material with thermo-sensitive for the enrichment and sensing of protein was successfully prepared by combining molecular imprinting technology with upconversion nanoparticles (UCNPs) and metal-organic frameworks (MOFs). Herein, the UCNPs acted as signal reporter for composite materials because of its excellent fluorescence property and chemical stability. MOFs were introduced to molecularly imprinted polymer (MIP) due to its high specific surface area which increases the rate of mass transfer relative to that of traditional bulk MIP. The thermo-sensitive imprinted material which allows for swelling and shrinking with response to temperature changes was prepared by choosing Bovine hemoglobin (BHB) as the template, N-isopropyl acrylamide (NIPAAM) as the temperature-sensitive functional monomer and N,N-methylenebisacrylamide (MBA) as the cross-linker. The recognition characterizations of imprinted material-coated UCNPs/MOFs (UCNPs/MOFs/MIP) were evaluated, and the results showed that the fluorescence intensity of UCNPs/MOFs/MIP reduced gradually with the increase of BHB concentration. The fluorescence material was response to the temperature. The adsorption capacity was as much as 167.6 mg/g at 28°C and 101.2mg/g at 44°C, which was higher than that of traditional MIP. Therefore, this new fluorescence material for enrichment and sensing protein is very promising for future applications.
一种新型荧光材料具有温度敏感性，可用于蛋白质的富集和传感，该材料通过将分子印迹技术与上转换纳米粒子（UCNPs）和金属有机骨架（MOFs）相结合成功制备。在此，由于其优异的荧光性能和化学稳定性，UCNPs 用作复合材料的信号报告器。MOFs 由于其高比表面积而被引入到分子印迹聚合物（MIP）中，这增加了相对于传统块状 MIP 的传质速率。选择牛血红蛋白（BHB）作为模板、N-异丙基丙烯酰胺（NIPAAM）作为温度敏感功能单体和 N,N-亚甲基双丙烯酰胺（MBA）作为交联剂，制备了对温度变化有溶胀和收缩响应的温敏印迹材料。评估了印迹材料涂覆的 UCNPs/MOFs（UCNPs/MOFs/MIP）的识别特性，结果表明，随着 BHB 浓度的增加，UCNPs/MOFs/MIP 的荧光强度逐渐降低。该荧光材料对温度有响应。在 28°C 时的吸附容量高达 167.6mg/g，在 44°C 时的吸附容量为 101.2mg/g，高于传统 MIP。因此，这种用于蛋白质富集和传感的新型荧光材料在未来的应用中具有很大的应用前景。
