Hospital de Clínicas de Porto Alegre (HCPA), Serviço de Otorrinolaringologia e Cirurgia Cérvico-Facial, Porto Alegre, RS, Brazil; Universidade Federal do Rio Grande do Sul (UFRGS), Programa de Pós-Graduação em Saúde da Criança e do Adolescente, Porto Alegre, RS, Brazil.
Universidade Federal do Rio Grande do Sul (UFRGS), Programa de Pós-Graduação em Saúde da Criança e do Adolescente, Porto Alegre, RS, Brazil; Hospital de Clínicas de Porto Alegre, Serviço de Pneumologia Pediátrica, Porto Alegre, RS, Brazil.
Braz J Otorhinolaryngol. 2023 Mar-Apr;89(2):254-263. doi: 10.1016/j.bjorl.2022.05.001. Epub 2022 May 20.
To evaluate the bacterial microbiome found in tracheostomy cannulas of a group of children diagnosed with glossoptosis secondary to Robin Sequence (RS), and its clinical implications.
Pediatric patients were enrolled in the study at the time of the cannula change in the hospital. During this procedure, the removed cannula was collected and stored for amplicon sequencing of 16s rRNA. DNA extraction was performed using DNeasy PowerBiofilm Kit (QIAGEN® ‒ Cat nº 24000-50) while sequencing was performed with the S5 (Ion S5™ System, Thermo Fisher Scientific), following Brazilian Microbiome Project (BMP) protocol.
All 12 patients included in the study were using tracheostomy uncuffed cannulas of the same brand, had tracheostomy performed for over 1-year and had used the removed cannula for approximately 3-months. Most abundant genera found were Aggregatibacter, Pseudomonas, Haemophilus, Neisseria, Staphylococcus, Fusobacterium, Moraxella, Streptococcus, Alloiococcus, and Capnocytophaga. Individual microbiome of each individual was highly variable, not correlating to any particular clinical characteristic.
The microbiome of tracheostomy cannulas is highly variable, even among patients with similar clinical characteristics, making it challenging to determine a standard for normality.
评估一组因 Robin 序列（RS）导致的悬雍垂后坠而被诊断为该病的儿童的气管造口管中发现的细菌微生物组及其临床意义。
在医院更换套管时，将儿科患者纳入研究。在此过程中，收集并储存取出的套管，用于 16s rRNA 的扩增子测序。使用 DNeasy PowerBiofilm 试剂盒（QIAGEN®-Cat nº 24000-50）进行 DNA 提取，而测序则使用巴西微生物组计划（BMP）协议下的 S5（Ion S5™ System，Thermo Fisher Scientific）进行。
所有纳入研究的 12 名患者均使用相同品牌的无囊气管造口管，气管造口术已超过 1 年，且已使用取出的套管约 3 个月。发现的最丰富的属是 Aggregatibacter、Pseudomonas、Haemophilus、Neisseria、Staphylococcus、Fusobacterium、Moraxella、Streptococcus、Alloiococcus 和 Capnocytophaga。每个个体的个体微生物组高度可变，与任何特定的临床特征均无相关性。
气管造口管的微生物组高度可变，即使在具有相似临床特征的患者中也是如此，这使得确定正常标准变得具有挑战性。
