State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, P. R. China.
School of Pharmacy, Guilin Medical University, Guilin, 541004, China.
Chem Biodivers. 2022 Jul;19(7):e202200449. doi: 10.1002/cbdv.202200449. Epub 2022 Jun 23.
Zanthoxylum nitidum (Roxb.) DC., is one of Guangxi's characteristic national medicines, and is the classic Laoban medicine of Yao people "Ru Shan Hu" and Zhuang medicine "Liang Bei Zhen". It has been used as an anti-inflammatory, analgesic and haemostatic medicine for thousands of years. In this study, four new sesquiterpenoids (1-4), along with six previously described coumarins (5-10), were isolated from 95 % EtOH extract of Zanthoxylum nitidum. Comprehensive spectroscopic analyses (NMR and HR-ESI-MS) were used to elucidate the structures of these isolates. The absolute configurations of nitidumine A-D (1-4) were established by electronic circular dichroism (ECD). Their cytotoxicity of all the isolates against five cancer cell lines (T24, HeLa, MGC-803, A549, and HepG2) was evaluated by MTT experiment and found not to be cytotoxicity.
荜拨（Zanthoxylum nitidum (Roxb.) DC.）是广西特色民族药之一，也是瑶医经典“如山虎”和壮药“两背针”的经典药物，具有几千年的抗炎、镇痛和止血作用。本研究从荜拨 95%乙醇提取物中分离得到四个新的倍半萜（1-4）和六个先前描述的香豆素（5-10）。综合光谱分析（NMR 和 HR-ESI-MS）用于阐明这些分离物的结构。通过电子圆二色性（ECD）确定了 nitidumine A-D（1-4）的绝对构型。通过 MTT 实验评价了所有分离物对五种癌细胞系（T24、HeLa、MGC-803、A549 和 HepG2）的细胞毒性，结果均无细胞毒性。
