State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, People's Republic of China.
Pharmaceutical Research Center and School of Chemistry and Chemical Engineering, Southeast University, Nanjing, Jiangsu 211189, People's Republic of China.
Fitoterapia. 2023 Jan;164:105381. doi: 10.1016/j.fitote.2022.105381. Epub 2022 Dec 7.
Five pairs of new racemic alkamides (1a/1b and 4a/4b-7a/7b) and two new achiral derivatives (2-3), as well as five known ones (8-12), were purified from the 95% EtOH extract of Zanthoxylum nitidum. Their structures were elucidated based on spectroscopic analyses (NMR and HR-ESI-MS), electronic circular dichroism (ECD) and NMR calculations. The enantiomeric separation was successfully achieved by chiral-phase HPLC-ECD measurements. Among all the isolates, compounds 2, 3, and 10 showed inhibitory effects against five human cancer cell lines, with IC values in range of 18.51-48.03 μM.
从两面针的 95%乙醇提取物中分离得到了 5 对新的外消旋烷酰胺（1a/1b 和 4a/4b-7a/7b）和 2 个新的非手性衍生物（2-3），以及 5 个已知化合物（8-12）。基于光谱分析（NMR 和 HR-ESI-MS）、电子圆二色性（ECD）和 NMR 计算，确定了它们的结构。通过手性相 HPLC-ECD 测量成功实现了对映体分离。在所有分离物中，化合物 2、3 和 10 对 5 个人类癌细胞系表现出抑制作用，IC 值范围为 18.51-48.03 μM。
