Tao Wenyang, Liu Wei, Wang Mingzhe, Zhou Wanyi, Xing Jianrong, Xu Jing, Pi Xionge, Wang Xiaotong, Lu Shengmin, Yang Ying
Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Foods. 2022 Jun 2;11(11):1641. doi: 10.3390/foods11111641.
is widely used as a health supplement, but its specific impact on healthy gut microbiota has not yet been clarified, nor has its impact on different human genders. To overcome the problems mentioned above. DOP was extracted and purified with an 8000-12,000 Da dialysis bag. The molecular weight and monosaccharide composition were determined using HPGPC and GC. Gas chromatography was used to detect the content of SCFA. 16S rDNA sequencing was used to analyze the diversity of human microbiota. The results showed that DOP contained two fractions, with an average molecular weight of 277 kDa and 1318 Da, and mainly composed of mannose and glucose. DOP can increase the relative abundance of benign microbiota and decrease the harmful types. Propionic acid content in women was significantly increased after DOP treatment. Finally, the correlation analysis revealed that DOP was beneficial to the microbiota of both men and women. It can be concluded from the results that DOP is a health supplement suitable for humans, and especially women.
它被广泛用作健康补充剂，但其对健康肠道微生物群的具体影响尚未阐明，对不同人类性别的影响也未明确。为克服上述问题，用8000 - 12,000 Da透析袋提取并纯化了DOP。使用高效凝胶渗透色谱法（HPGPC）和气相色谱法（GC）测定分子量和单糖组成。用气相色谱法检测短链脂肪酸（SCFA）含量。采用16S核糖体DNA（rDNA）测序分析人类微生物群的多样性。结果表明，DOP含有两个组分，平均分子量分别为277 kDa和1318 Da，主要由甘露糖和葡萄糖组成。DOP可增加良性微生物群的相对丰度，减少有害类型。DOP处理后女性体内丙酸含量显著增加。最后，相关性分析表明DOP对男性和女性的微生物群均有益。从结果可以得出结论，DOP是一种适合人类尤其是女性的健康补充剂。
