Dieli F, Salerno A
Cell Immunol. 1987 Apr 1;105(2):386-96. doi: 10.1016/0008-8749(87)90086-4.
Lymph node cells collected from CBA/J mice 4 days after painting the skin with picryl chloride are able to immunize naive recipients by hapten-IgM immuno complexes. These cells ("4-day" cells) activate the early components of the classical pathway of complement from mice of the H-2 Sd haplotype (high-C4), but fail to activate the classical pathway of complement from mice of the H-2 Sk haplotype (low-C4). Incubation of "4-day" cells in complement from mice with high-C4 levels abolishes the induction of contact sensitivity, probably as a consequence of the solubilization of membrane-bound immuno complexes caused by complement activation. The presence of "4-day" cells is determined by the levels of C4. In fact, using strains of mice which differ only at the S region of the H-2 complex, we found that mice of the H-2 Sd (and perhaps H-2 Sb) haplotype (high-C4 levels) lack "4-day" cells in their lymph nodes and this is due to the activation of the early components of the classical complement pathway which occurs in vivo in these mice during sensitization with picryl chloride. The finding that contact sensitivity reaction to picryl chloride in H-2 Sk mice lasts about 21 days, whereas H-2 Sd mice show a contact sensitivity reaction until 7 days after sensitization, strongly suggests that the S region, and in particular C4 levels, controls the persistence of "4-day" immunogenic cells, and so play a role in the duration of the contact sensitivity reaction to picryl chloride in the mouse.
在用苦味酸氯涂抹皮肤4天后从CBA/J小鼠收集的淋巴结细胞，能够通过半抗原-IgM免疫复合物使未致敏的受体产生免疫。这些细胞（“4天”细胞）能激活H-2 Sd单倍型（高C4）小鼠经典补体途径的早期成分，但不能激活H-2 Sk单倍型（低C4）小鼠的经典补体途径。将“4天”细胞与高C4水平小鼠的补体一起孵育，可消除接触敏感性的诱导，这可能是补体激活导致膜结合免疫复合物溶解的结果。“4天”细胞的存在取决于C4水平。事实上，使用仅在H-2复合体的S区域不同的小鼠品系，我们发现H-2 Sd（可能还有H-2 Sb）单倍型（高C4水平）的小鼠在其淋巴结中缺乏“4天”细胞，这是由于在用苦味酸氯致敏期间，这些小鼠体内发生了经典补体途径早期成分的激活。H-2 Sk小鼠对苦味酸氯的接触敏感性反应持续约21天，而H-2 Sd小鼠在致敏后7天内都表现出接触敏感性反应，这一发现强烈表明，S区域，特别是C4水平，控制着“4天”免疫原性细胞的持久性，因此在小鼠对苦味酸氯的接触敏感性反应持续时间中发挥作用。
