Salerno A, Brai M, Dieli F, Colonna Romano G, Abrignani S, Colizzi V, Asherson G L
Cell Immunol. 1984 Dec;89(2):376-84. doi: 10.1016/0008-8749(84)90339-3.
Lymph node cells collected 4 days after painting the skin with picryl chloride activate the first components of the classical pathway of complement cascade, as shown by consumption of C4 of rabbit complement with total sparing of C5 and factor B activity. In contrast, lymph node cells collected 1 or 6 days after sensitization fail to do so. The ability of "4-day" cells to activate complement is inhibited by treating the cells with specific low-molecular-weight hapten, which is known to dissociate the immune complex present on the cell surface. When mouse serum was used as source of complement, a different behavior in complement activation between CBA/J and B10.D2-New/SnJ serum was observed: "4-day" cells failed to consume CBA/J serum whereas a normal complement activation was detected when B10.D2-New/SnJ serum was used. Using these two sera which differ in the level of C4, an inverse relationship between the ability of "4-day" cells to activate complement and their capacity to induce contact sensitivity when injected into the footpad of normal recipients was reported. Experiments performed using sera from C5 genetically deficient mice demonstrate that only the early complement components are involved, suggesting that membrane immune complexes are solubilized as a result of complement activation; on the other hand, membrane bound activated complement components could alter the immunizing potential of "4-day" cells.
在用苦味酸氯涂抹皮肤4天后收集的淋巴结细胞可激活补体级联经典途径的首个成分，兔补体C4的消耗表明了这一点，而C5和B因子活性则完全保留。相比之下，致敏后1天或6天收集的淋巴结细胞则无法激活。用特定的低分子量半抗原处理细胞可抑制“4天”细胞激活补体的能力，已知该半抗原可解离细胞表面存在的免疫复合物。当使用小鼠血清作为补体来源时，观察到CBA/J和B10.D2-New/SnJ血清在补体激活方面存在不同行为：“4天”细胞无法消耗CBA/J血清，而使用B10.D2-New/SnJ血清时则检测到正常的补体激活。使用这两种C4水平不同的血清，报告了“4天”细胞激活补体的能力与其注入正常受体足垫时诱导接触敏感性的能力之间呈负相关。使用来自C5基因缺陷小鼠的血清进行的实验表明，只有早期补体成分参与其中，这表明膜免疫复合物因补体激活而溶解；另一方面，膜结合的活化补体成分可能会改变“4天”细胞的免疫潜力。
