IVI-RMA Seville, Avda. Américo Vespucio 19, 41092 Seville, Spain.
IVI Foundation, Instituto de Investigación Sanitaria La Fe (IIS La Fe), 46026 Valencia, Spain.
Cells. 2022 May 30;11(11):1794. doi: 10.3390/cells11111794.
Clinical outcome in assisted reproduction techniques (ARTs) is mainly influenced by the quality of gametes used. It is known that a high percentage of sperm DNA fragmentation (DNAf) decreases the success of ART clinical results. Therefore, techniques such as magnetic-activated cell sorting (MACS) help to improve results in cases of patients with a high percentage of DNAf. Cryopreservation of sperm in donor intrauterine insemination (D-IUI) treatments increases sperm DNAf, so patients using these sperm samples can benefit from using this technique. This prospective randomized national multicenter study analyzed clinical outcomes of 181 D-IUI treatments. MACS was performed after density gradient centrifugation (DGC) in 90 thawed semen donor samples (MACSG), whereas only DGC was performed in 91 thawed semen donor samples (CG). To our knowledge, this is the first study analyzing the effect of MACS on D-IUI cycles. Our results show no significant differences in gestation, live birth, or miscarriage rates between the two groups. We believe that further studies with a larger sample size are needed to evaluate the application of MACS in combination with standard IUI donor sperm preparations in fertility clinics.
辅助生殖技术（ART）的临床结局主要受所用配子质量的影响。众所周知，高比例的精子 DNA 碎片化（DNAf）会降低 ART 临床结果的成功率。因此，磁激活细胞分选（MACS）等技术有助于改善 DNAf 比例较高患者的治疗结果。在供体宫腔内人工授精（D-IUI）治疗中冷冻精子会增加精子 DNAf，因此使用这些精子样本的患者可以受益于使用该技术。这项前瞻性随机全国多中心研究分析了 181 例 D-IUI 治疗的临床结局。在 90 例解冻的精液供体样本（MACSG）中进行密度梯度离心（DGC）后进行 MACS，而在 91 例解冻的精液供体样本（CG）中仅进行 DGC。据我们所知，这是首次分析 MACS 对 D-IUI 周期影响的研究。我们的结果显示两组之间的妊娠、活产或流产率没有显著差异。我们认为需要进行更大样本量的进一步研究，以评估 MACS 在生育诊所中与标准 IUI 供体精子制剂联合应用的效果。
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