Gynecology and Obstetrics Ward with Gynecologic Oncology Subdivision, J. Śniadecki's Specialistic Hospital, 33-300 Nowy Sącz, Poland.
Department of Pathophysiology, Jagiellonian University Medical College, 31-121 Krakow, Poland.
Int J Mol Sci. 2022 May 31;23(11):6155. doi: 10.3390/ijms23116155.
Oxygen balance is crucial for angiogenesis, immunity, and tissue repair. The human oviduct is essential for reproductive function, and any imbalance in homeostasis leads to fertility disturbances and might be a reason for ectopic pregnancy development. Uterine myoma is a widespread benign tumour, which is often accompanied by infertility. Telocytes have been discussed in the contexts of motility, fibrosis development, and angiogenesis. We observed the oviducts from patients with and without uterine myoma, comparing the expression of HIF-1, HO, VEGF and its receptor, NOS, oestrogen, and progesterone receptors by immunolabeling. The myometrial and oviductal telocytes were also compared in both groups. Biochemical analyses were conducted for FSH, LH, AMH, sFlt, oestrogen, and progesterone in blood samples. Patients with uterine myoma have different expressions of sex steroid receptors and an increased number of telocytes. The decreasing VEFG expression was compensated by the rise in the HIF-1 and NOS expression. Blood biochemical analyses revealed a higher progesterone level and lower AMH in patients with uterine myoma. No differences in sFlt, FSH, and LF were observed. Uterine myoma impacts oviduct oxygen homeostasis and might cause fertility disturbances (uterine and oviductal infertility factors).
氧平衡对于血管生成、免疫和组织修复至关重要。人类输卵管对于生殖功能至关重要，任何体内平衡的失衡都会导致生育障碍，并且可能是异位妊娠发展的原因。子宫肌瘤是一种广泛存在的良性肿瘤，常伴有不孕。人们已经讨论过间质细胞在运动、纤维化发展和血管生成中的作用。我们通过免疫标记观察了有和没有子宫肌瘤的患者的输卵管，比较了 HIF-1、HO、VEGF 及其受体、NOS、雌激素和孕激素受体的表达。还比较了两组患者的子宫肌层和输卵管间质细胞。对血液样本中的 FSH、LH、AMH、sFlt、雌激素和孕激素进行了生化分析。患有子宫肌瘤的患者的性激素受体表达不同，间质细胞数量增加。VEGF 表达的减少被 HIF-1 和 NOS 表达的增加所补偿。血液生化分析显示，患有子宫肌瘤的患者孕激素水平较高，AMH 水平较低。未观察到 sFlt、FSH 和 LF 的差异。子宫肌瘤会影响输卵管的氧平衡，并可能导致生育障碍（子宫和输卵管不孕因素）。
