Department of Pharmaceutical Biochemistry, Faculty of Pharmacy, Medical University of Gdansk, Debinki, 80-211 Gdansk, Poland.
Nutrients. 2022 May 31;14(11):2310. doi: 10.3390/nu14112310.
Recently, we have demonstrated a decreased level of iso-branched-chain fatty acids (iso-BCFAs) in patients with excessive weight. However, it is still unclear whether BCFAs may influence lipid metabolism and inflammation in lipogenic tissues. To verify this, human visceral adipocytes were cultured with three different concentrations of selected iso-BCFA (14-methylpentadecanoic acid) and anteiso-BCFA (12-methyltetradecanoic acid), and then the expression of genes associated with lipid metabolism (-fatty acid synthase; -sterol regulatory element-binding protein 1; -stearoyl-CoA desaturase; -fatty acid elongase 4; -fatty acid elongase 6; -fatty acid desaturase 2; -fatty acid desaturase 1) and inflammation (-cyclooxygenase 2; -lipoxygenase 15; -interleukin 6) were determined. This study demonstrates for the first time that incubation with iso-BCFA decreases the expression of adipocyte genes that are associated with lipid metabolism (except ) and inflammation. These findings suggest that changes in the iso-BCFA profile in obese patients may contribute to adipose inflammation and dyslipidemia. Further studies should evaluate whether iso-BCFA supplementation in obese patients would be beneficial.
最近，我们在超重患者中发现异支链脂肪酸（iso-BCFAs）水平降低。然而，BCFAs 是否会影响脂肪生成组织中的脂质代谢和炎症仍不清楚。为了验证这一点，我们用三种不同浓度的选定异支链脂肪酸（14-甲基十五烷酸）和 anteiso-BCFA（12-甲基十四烷酸）培养人内脏脂肪细胞，然后测定与脂质代谢（脂肪酸合成酶；固醇调节元件结合蛋白 1；硬脂酰辅酶 A 去饱和酶；脂肪酸延长酶 4；脂肪酸延长酶 6；脂肪酸去饱和酶 2；脂肪酸去饱和酶 1）和炎症（环氧化酶 2；脂加氧酶 15；白细胞介素 6）相关的基因表达。这项研究首次表明，异支链脂肪酸孵育会降低与脂质代谢（除外）和炎症相关的脂肪细胞基因的表达。这些发现表明，肥胖患者异支链脂肪酸谱的变化可能导致脂肪组织炎症和血脂异常。进一步的研究应评估肥胖患者补充异支链脂肪酸是否有益。
