Yuan Jiazheng, O'Neal Jordan, Brown Daria, Zhu Lieceng
Plant Interaction Laboratory, Department of Biological and Forensic Sciences, Fayetteville State University, Fayetteville, NC 28301, USA.
Plants (Basel). 2022 May 25;11(11):1402. doi: 10.3390/plants11111402.
Heat stress compromises wheat () resistance to Hessian fly (HF, (Say)). This study aimed to investigate the impact of heat stress on transcript expression of wheat genes associated with resistance to HF infestation under normal and heat-stressed conditions. To this end, 'Molly', a wheat cultivar containing the resistance gene , was subjected to HF infestation, heat stress, and the combination of HF infestation and heat stress. Our RNA-Seq approach identified 21 wheat genes regulated by HF infestation under normal temperatures (18 °C) and 155 genes regulated by HF infestation when plants were exposed to 35 °C for 6 h. Three differentially expressed genes (DEGs) from the RNA-Seq analysis were selected to validate the gene function of these DEGs using the RT-qPCR approach, indicating that these DEGs may differentially contribute to the expression of wheat resistance during the early stage of wheat-HF interaction under various stresses. Moreover, the jasmonate ZIM domain (JAZ) gene was also significantly upregulated under these treatments. Our results suggest that the genes in heat-stressed wheat plants are more responsive to HF infestation than those in plants growing under normal temperature conditions, and these genes in HF-infested wheat plants are more responsive to heat stress than those in plants without infestation.
热胁迫会削弱小麦对黑森瘿蚊（HF，Mayetiola destructor (Say)）的抗性。本研究旨在调查在正常和热胁迫条件下，热胁迫对与小麦抗HF侵染相关基因转录表达的影响。为此，对含有抗性基因的小麦品种‘莫莉’进行了HF侵染、热胁迫以及HF侵染与热胁迫相结合的处理。我们的RNA测序方法鉴定出在正常温度（18℃）下受HF侵染调控的21个小麦基因，以及当植株在35℃下处理6小时时受HF侵染调控的155个基因。从RNA测序分析中选择了三个差异表达基因（DEG），使用RT-qPCR方法验证这些DEG的基因功能，这表明在各种胁迫下，这些DEG在小麦与HF互作早期可能对小麦抗性表达有不同贡献。此外，在这些处理下，茉莉酸ZIM结构域（JAZ）基因也显著上调。我们的结果表明，热胁迫下的小麦植株中的基因比正常温度条件下生长的植株中的基因对HF侵染更敏感，并且受HF侵染的小麦植株中的这些基因比未受侵染的植株中的基因对热胁迫更敏感。
