Chao Lei, Pan Zhenyuan, Wang Jing, Wu Yuanlong, Shui Guangling, Aini Nurimanguli, Tang Binghui, Guo Chunping, Han Peng, Shao Panxia, Tian Xiaomin, Chang Xinyi, An Qiushuang, Ma Chunmei, You Chunyuan, Zhu Longfu, Nie Xinhui
Key Laboratory of Oasis Ecology Agricultural of Xinjiang Production and Construction Corps, Agricultural College, Shihezi University, Shihezi 832003, China.
College of Informatics, Huazhong Agricultural University, Wuhan 430070, China.
Plants (Basel). 2022 May 31;11(11):1483. doi: 10.3390/plants11111483.
With the promotion and popularization of machine cotton-picking, more and more attention has been paid to the selection of early-maturity varieties with compact plant architecture. The type of fruit branch is one of the most important factors affecting plant architecture and early maturity of cotton. Heredity analysis of the cotton fruit branch is beneficial to the breeding of machine-picked cotton. Phenotype analysis showed that the types of fruit branches in cotton are controlled by a single recessive gene. Using an F population crossed with Huaxin102 (normal branch) and 04N-11 (nulliplex branch), BSA (Bulked Segregant Analysis) resequencing analysis and gene cloning in 04N-11, and allelic testing, showed that fruit branch type was controlled by the gene, located on chromosome D07. , a new recessive genotype of , was found in 04N-11. Through candidate gene association analysis, SNP 20_15811516_SNV was found to be associated with plant architecture and early maturity in the Xinjiang natural population. The gene, which is related to early maturity and the plant architecture of cotton, is a branch-type gene of cotton. The 20_15811516_SNV marker, obtained from the Xinjiang natural population, was used for the assisted breeding of machine-picked cotton varieties.
随着机采棉的推广普及，株型紧凑的早熟品种选育越来越受到重视。果枝类型是影响棉花株型和早熟性的重要因素之一。对棉花果枝进行遗传分析有利于机采棉育种。表型分析表明，棉花果枝类型受一对隐性基因控制。以华新102（正常果枝）和04N - 11（无效果枝）杂交构建F群体，对04N - 11进行混合分组分析法（BSA）重测序分析、基因克隆及等位性检测，结果表明果枝类型受位于D07染色体上的基因控制。在04N - 11中发现了一种新的隐性基因型。通过候选基因关联分析，在新疆自然群体中发现SNP 20_15811516_SNV与株型和早熟性相关。该与棉花早熟性和株型相关的基因是棉花的一个果枝类型基因。利用从新疆自然群体中获得的20_15811516_SNV标记，进行机采棉品种的辅助育种。
