• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

[植物名称]中钙调蛋白/类钙调蛋白（CAM/CML）家族的特征，以及一个异位表达基因（[基因名称]）对转基因[植物名称]幼苗和果实发育的影响

Characterization of the Calmodulin/Calmodulin-like Protein (CAM/CML) Family in , and the Influence of an Ectopically Expressed Gene () on Seedling and Fruit Development of Transgenic .

作者信息

Zhang Xinxin, Tian Juan, Li Sai, Liu Yuying, Feng Ting, Wang Yunyun, Li Yuanjin, Huang Xinxin, Li Dahui

机构信息

College of Life Sciences, Anhui Agricultural University, Hefei 230036, China.

出版信息

Plants (Basel). 2022 Jun 4;11(11):1506. doi: 10.3390/plants11111506.

DOI:10.3390/plants11111506
PMID:35684283
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9183014/
Abstract

Calmodulins (CAMs) and calmodulin-like proteins (CMLs) can participate in the regulation of various physiological processes via sensing and decoding Ca signals. To reveal the characteristics of the CAM/CML family in , a comprehensive analysis was performed at the genome-wide level. A total of 26 s, consisting of 5 s and 21 s, was identified on 11 out of 12 chromosomes in . They displayed a certain degree of multiplicity in their sequences, albeit with conserved EF hands. Collinearity analysis suggested that tandem rather than segmental or whole-genome duplications were likely to play roles in the evolution of the family. Furthermore, s were grouped into higher, lower, and moderate expression in magnitude. The cis-acting regulatory elements involved in phytohormone-responsiveness within promotors may explain their varied expression profiles. The ectopic expression of a gene () in transgenic led to phenotypes with significantly shortened root length and seedling height, and decreased yields of both pods and seeds. Moreover, an electrophoresis mobility shift assay demonstrated the Ca-binding activity of Gb_30819 in vitro. Altogether, these results contribute to insights into the characteristics of the evolution and expression of s, as well as evidence for Ca-CAM/CML pathways functioning within the ancient gymnosperm .

摘要

钙调蛋白（CAMs）和类钙调蛋白（CMLs）可通过感知和解码钙信号参与多种生理过程的调控。为揭示[具体物种名称]中CAM/CML家族的特征，在全基因组水平上进行了综合分析。在[具体物种名称]的12条染色体中的11条上共鉴定出26个[具体基因名称]，包括5个[具体基因名称]和21个[具体基因名称]。它们的序列显示出一定程度的多样性，尽管具有保守的EF手结构域。共线性分析表明，串联重复而非片段重复或全基因组重复可能在[具体基因名称]家族的进化中起作用。此外，[具体基因名称]在表达量上被分为高、低和中等表达。[具体基因名称]启动子内参与植物激素应答的顺式作用调控元件可能解释了它们不同的表达谱。一个[具体基因名称]基因（Gb_30819）在转基因[具体植物名称]中的异位表达导致根长和幼苗高度显著缩短，以及豆荚和种子产量下降的表型。此外，电泳迁移率变动分析在体外证明了Gb_30819的钙结合活性。总之，这些结果有助于深入了解[具体基因名称]的进化和表达特征，以及古代裸子植物[具体物种名称]中钙 - CAM/CML途径发挥作用的证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/2c1057a1c296/plants-11-01506-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/f42ecbd7f09c/plants-11-01506-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/a686649f1fb5/plants-11-01506-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/0a996b28d5e0/plants-11-01506-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/28a9e85518b0/plants-11-01506-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/2b74a405f9bc/plants-11-01506-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/1247193b5be5/plants-11-01506-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/9ef0d4e120e1/plants-11-01506-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/28a7c62518c0/plants-11-01506-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/924b90aebf48/plants-11-01506-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/2c1057a1c296/plants-11-01506-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/f42ecbd7f09c/plants-11-01506-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/a686649f1fb5/plants-11-01506-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/0a996b28d5e0/plants-11-01506-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/28a9e85518b0/plants-11-01506-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/2b74a405f9bc/plants-11-01506-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/1247193b5be5/plants-11-01506-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/9ef0d4e120e1/plants-11-01506-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/28a7c62518c0/plants-11-01506-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/924b90aebf48/plants-11-01506-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfaa/9183014/2c1057a1c296/plants-11-01506-g010.jpg

相似文献

1
Characterization of the Calmodulin/Calmodulin-like Protein (CAM/CML) Family in , and the Influence of an Ectopically Expressed Gene () on Seedling and Fruit Development of Transgenic .[植物名称]中钙调蛋白/类钙调蛋白（CAM/CML）家族的特征，以及一个异位表达基因（[基因名称]）对转基因[植物名称]幼苗和果实发育的影响
Plants (Basel). 2022 Jun 4;11(11):1506. doi: 10.3390/plants11111506.
2
Genomics and evolutionary aspect of calcium signaling event in calmodulin and calmodulin-like proteins in plants.植物中钙调蛋白及类钙调蛋白中钙信号事件的基因组学与进化方面
BMC Plant Biol. 2017 Feb 3;17(1):38. doi: 10.1186/s12870-017-0989-3.
3
Evolution and diversification of CaM/CML gene family in green plants.钙调蛋白/CML 基因家族在绿色植物中的进化和多样化。
Plant Physiol Biochem. 2023 Sep;202:107922. doi: 10.1016/j.plaphy.2023.107922. Epub 2023 Aug 10.
4
Genome-wide identification and expression analysis of CaM/CML genes in Brassica napus under abiotic stress.甘蓝型油菜非生物胁迫下钙调素/CML 基因的全基因组鉴定和表达分析。
J Plant Physiol. 2020 Dec;255:153251. doi: 10.1016/j.jplph.2020.153251. Epub 2020 Aug 14.
5
Genome-wide identification, and phylogenetic and expression profiling analyses of CaM and CML genes in Brassica rapa and Brassica oleracea.甘蓝型油菜和白菜基因组中钙调素和钙调蛋白激酶基因的全基因组鉴定、系统进化和表达谱分析。
Gene. 2018 Nov 30;677:232-244. doi: 10.1016/j.gene.2018.07.038. Epub 2018 Jul 17.
6
Genome-Wide Identification and Expression Analysis of (CaM) and (CML) Genes in the Brown Algae .褐藻中钙调蛋白（CaM）和钙调蛋白类似蛋白（CML）基因的全基因组鉴定与表达分析
Plants (Basel). 2023 May 9;12(10):1934. doi: 10.3390/plants12101934.
7
Genome-wide identification and expression analysis of calmodulin and calmodulin-like genes in apple (Malus × domestica).苹果（Malus × domestica）钙调蛋白和钙调蛋白样基因的全基因组鉴定和表达分析。
Plant Physiol Biochem. 2019 Jun;139:600-612. doi: 10.1016/j.plaphy.2019.04.014. Epub 2019 Apr 17.
8
Calmodulins and related potential calcium sensors of Arabidopsis.拟南芥的钙调蛋白及相关潜在钙传感器
New Phytol. 2003 Sep;159(3):585-598. doi: 10.1046/j.1469-8137.2003.00845.x.
9
Identification, characterization, and expression analysis of calmodulin and calmodulin-like genes in grapevine (Vitis vinifera) reveal likely roles in stress responses.葡萄（Vitis vinifera）钙调蛋白和钙调蛋白样基因的鉴定、特征分析和表达分析揭示了其在应激反应中的可能作用。
Plant Physiol Biochem. 2018 Aug;129:221-237. doi: 10.1016/j.plaphy.2018.06.003. Epub 2018 Jun 4.
10
and gene family in barley: Identification, characterization and expression analyses.大麦中的基因家族：鉴定、特征分析及表达分析。
Front Plant Sci. 2022 Aug 19;13:964888. doi: 10.3389/fpls.2022.964888. eCollection 2022.

引用本文的文献

1
Developmental Morphology, Physiology, and Molecular Basis of the Pentagram Fruit of ..的五角星果实的发育形态学、生理学及分子基础
Plants (Basel). 2024 Sep 26;13(19):2696. doi: 10.3390/plants13192696.
2
Genome-wide identification and expression analysis of calmodulin and calmodulin-like genes in passion fruit (Passiflora edulis) and their involvement in flower and fruit development.百香果（Passiflora edulis）钙调蛋白和钙调蛋白样基因的全基因组鉴定和表达分析及其在花和果实发育中的作用。
BMC Plant Biol. 2024 Jul 3;24(1):626. doi: 10.1186/s12870-024-05295-y.
3
Genome-Wide Identification and Expression Analysis of Calmodulin-Like Gene Family in Revealed Their Role in Response to Salt and Cold Stress.

本文引用的文献

1
Calmodulins and related potential calcium sensors of Arabidopsis.拟南芥的钙调蛋白及相关潜在钙传感器
New Phytol. 2003 Sep;159(3):585-598. doi: 10.1046/j.1469-8137.2003.00845.x.
2
Calmodulin as a versatile calcium signal transducer in plants.钙调蛋白作为植物中一种多功能的钙信号转导蛋白。
New Phytol. 2001 Jul;151(1):35-66. doi: 10.1046/j.1469-8137.2001.00154.x.
3
Unravelling response-specificity in Ca signalling pathways in plant cells.解析植物细胞钙信号通路中的反应特异性
基因组范围内钙调素样基因家族的鉴定与表达分析揭示了它们在响应盐胁迫和冷胁迫中的作用。
Curr Issues Mol Biol. 2023 Feb 16;45(2):1693-1711. doi: 10.3390/cimb45020109.
4
Genome-wide identification and expression analysis of calmodulin-like proteins in cucumber.黄瓜钙调素样蛋白的全基因组鉴定和表达分析。
PeerJ. 2023 Jan 13;11:e14637. doi: 10.7717/peerj.14637. eCollection 2023.
New Phytol. 2001 Jul;151(1):7-33. doi: 10.1046/j.1469-8137.2001.00173.x.
4
Genome-wide identification and expression analysis of calmodulin and calmodulin-like genes in apple (Malus × domestica).苹果（Malus × domestica）钙调蛋白和钙调蛋白样基因的全基因组鉴定和表达分析。
Plant Physiol Biochem. 2019 Jun;139:600-612. doi: 10.1016/j.plaphy.2019.04.014. Epub 2019 Apr 17.
5
Evolutionary and functional analysis of the plant-specific NADPH oxidase gene family in L.番茄中植物特异性NADPH氧化酶基因家族的进化与功能分析 （注：原英文中“L.”指代不明，这里按“番茄（Lycopersicon）”推测翻译，如果不是番茄请根据实际情况调整）
R Soc Open Sci. 2019 Feb 27;6(2):181727. doi: 10.1098/rsos.181727. eCollection 2019 Feb.
6
Transcriptomic profiling identifies differentially expressed genes associated with programmed cell death of nucellar cells in Ginkgo biloba L.转录组谱分析鉴定与银杏核细胞程序性死亡相关的差异表达基因。
BMC Plant Biol. 2019 Feb 28;19(1):91. doi: 10.1186/s12870-019-1671-8.
7
Identification, characterization, and expression analysis of calmodulin and calmodulin-like genes in grapevine (Vitis vinifera) reveal likely roles in stress responses.葡萄（Vitis vinifera）钙调蛋白和钙调蛋白样基因的鉴定、特征分析和表达分析揭示了其在应激反应中的可能作用。
Plant Physiol Biochem. 2018 Aug;129:221-237. doi: 10.1016/j.plaphy.2018.06.003. Epub 2018 Jun 4.
8
Genome-wide identification and expression analysis of calmodulin-like (CML) genes in Chinese cabbage (Brassica rapa L. ssp. pekinensis).白菜（甘蓝亚种 Brassica rapa L. ssp. pekinensis）钙调素样蛋白（CML）基因的全基因组鉴定与表达分析。
BMC Genomics. 2017 Nov 2;18(1):842. doi: 10.1186/s12864-017-4240-2.
9
Analysis of EF-Hand Proteins in Soybean Genome Suggests Their Potential Roles in Environmental and Nutritional Stress Signaling.大豆基因组中EF-手型蛋白的分析表明它们在环境和营养胁迫信号传导中的潜在作用。
Front Plant Sci. 2017 May 24;8:877. doi: 10.3389/fpls.2017.00877. eCollection 2017.
10
Overexpression of OsERF48 causes regulation of OsCML16, a calmodulin-like protein gene that enhances root growth and drought tolerance.OsERF48的过表达导致对OsCML16的调控，OsCML16是一种类钙调蛋白基因，可增强根系生长和耐旱性。
Plant Biotechnol J. 2017 Oct;15(10):1295-1308. doi: 10.1111/pbi.12716. Epub 2017 Mar 27.