Cai Kangfeng, Kuang Liuhui, Yue Wenhao, Xie Shanggeng, Xia Xue, Zhang Guoping, Wang Junmei
Key Laboratory of Digital Dry Land Crops of Zhejiang Province, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.
National Barley Improvement Centre, Hangzhou, China.
Front Plant Sci. 2022 Aug 19;13:964888. doi: 10.3389/fpls.2022.964888. eCollection 2022.
Calmodulin (CaM) and calmodulin-like (CML) proteins are Ca relays and play diverse and multiple roles in plant growth, development and stress responses. However, gene family has not been identified in barley (). In the present study, 5 and 80 were identified through a genome-wide analysis. All HvCaM proteins possessed 4 EF-hand motifs, whereas HvCMLs contained 1 to 4 EF-hand motifs. , and coded the same polypeptide although they differed in nucleotide sequence, which was identical to the polypeptides coded by , and . were unevenly distributed over barley 7 chromosomes, and could be phylogenetically classified into 8 groups. differed in gene structure, -acting elements and tissue expression patterns. Segmental and tandem duplication were observed among during evolution. , , and were dispensable genes and the others were core genes in barley pan-genome. In addition, 14 genes were selected to examine their responses to salt, osmotic and low potassium stresses by qRT-PCR, and their expression were stress-and time-dependent. These results facilitate our understanding and further functional identification of .
钙调蛋白（CaM）和类钙调蛋白（CML）是钙信号传递蛋白，在植物生长、发育和应激反应中发挥着多样且多重的作用。然而，尚未在大麦中鉴定出该基因家族。在本研究中，通过全基因组分析鉴定出了5个HvCaM和80个HvCML。所有HvCaM蛋白均具有4个EF-手型基序，而HvCMLs含有1至4个EF-手型基序。HvCaM1;1、HvCaM1;2和HvCaM1;3编码相同的多肽，尽管它们在核苷酸序列上有所不同，这与HvCaM2;1、HvCaM2;2和HvCaM2;3编码的多肽相同。HvCMLs不均匀地分布在大麦的7条染色体上，并且在系统发育上可分为8组。HvCMLs在基因结构、顺式作用元件和组织表达模式方面存在差异。在进化过程中，观察到HvCMLs之间存在片段重复和串联重复。HvCML13、HvCML17、HvCML20和HvCML24是大麦泛基因组中的可有可无基因，其他的是核心基因。此外，选择了14个HvCML基因通过qRT-PCR检测它们对盐、渗透和低钾胁迫的反应，其表达具有胁迫和时间依赖性。这些结果有助于我们对HvCMLs的理解和进一步的功能鉴定。
