Biochemistry Department, Science College, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS Route de Soukra, Université de Sfax-Tunisia, Sfax 3038, Tunisia.
Molecules. 2022 May 26;27(11):3437. doi: 10.3390/molecules27113437.
Secretory group V phospholipase A2 (PLA-V) is known to be involved in inflammatory processes in cellular studies, nevertheless, the biochemical and the enzymatic characteristics of this important enzyme have been unclear yet. We reported, as a first step towards understanding the biochemical properties, catalytic characteristics, antimicrobial and cytotoxic effects of this PLA, the production of PLA-V from dromedary. The obtained DrPLA-V has an absolute requirement for Ca and NaTDC for enzymatic activity with an optimum pH of 9 and temperature of 45 °C with phosphatidylethanolamine as a substrate. Kinetic parameters showed that / is 2.6 ± 0.02 mM s. The enzyme was found to display potent Gram-positive bactericidal activity (with IC50 values of about 5 µg/mL) and antifungal activity (with IC50 values of about 25 µg/mL)in vitro. However, the purified enzyme did not display a cytotoxic effect against cancer cells.
分泌型 PLA2（PLA-V）在细胞研究中参与炎症反应，然而，这种重要酶的生化和酶学特性仍不清楚。我们报道了作为理解该 PLA 生化特性、催化特性、抗菌和细胞毒性作用的第一步，从单峰驼中提取 PLA-V。所得的 DrPLA-V 的酶活性对 Ca 和 NaTDC 有绝对要求，最适 pH 为 9，最适温度为 45°C，以磷脂酰乙醇胺为底物。动力学参数表明 K m 为 2.6 ± 0.02 mM。该酶在体外显示出对革兰氏阳性菌有很强的杀菌活性（IC50 值约为 5 µg/mL）和抗真菌活性（IC50 值约为 25 µg/mL）。然而，纯化后的酶对癌细胞没有细胞毒性作用。
