从单峰驼中提取的 V 组磷酸脂酶 A2 的生化、动力学和生物学特性
Biochemical, Kinetic and Biological Properties of Group V Phospholipase A2 from Dromedary.
机构信息
Biochemistry Department, Science College, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS Route de Soukra, Université de Sfax-Tunisia, Sfax 3038, Tunisia.
出版信息
Molecules. 2022 May 26;27(11):3437. doi: 10.3390/molecules27113437.
Secretory group V phospholipase A2 (PLA-V) is known to be involved in inflammatory processes in cellular studies, nevertheless, the biochemical and the enzymatic characteristics of this important enzyme have been unclear yet. We reported, as a first step towards understanding the biochemical properties, catalytic characteristics, antimicrobial and cytotoxic effects of this PLA, the production of PLA-V from dromedary. The obtained DrPLA-V has an absolute requirement for Ca and NaTDC for enzymatic activity with an optimum pH of 9 and temperature of 45 °C with phosphatidylethanolamine as a substrate. Kinetic parameters showed that / is 2.6 ± 0.02 mM s. The enzyme was found to display potent Gram-positive bactericidal activity (with IC50 values of about 5 µg/mL) and antifungal activity (with IC50 values of about 25 µg/mL)in vitro. However, the purified enzyme did not display a cytotoxic effect against cancer cells.
分泌型 PLA2（PLA-V）在细胞研究中参与炎症反应，然而，这种重要酶的生化和酶学特性仍不清楚。我们报道了作为理解该 PLA 生化特性、催化特性、抗菌和细胞毒性作用的第一步，从单峰驼中提取 PLA-V。所得的 DrPLA-V 的酶活性对 Ca 和 NaTDC 有绝对要求，最适 pH 为 9，最适温度为 45°C，以磷脂酰乙醇胺为底物。动力学参数表明 K m 为 2.6 ± 0.02 mM。该酶在体外显示出对革兰氏阳性菌有很强的杀菌活性（IC50 值约为 5 µg/mL）和抗真菌活性（IC50 值约为 25 µg/mL）。然而，纯化后的酶对癌细胞没有细胞毒性作用。
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