利用代谢组学和生物活性分析相结合的策略对 L. 的商业材料进行生物勘探。

Towards Bioprospection of Commercial Materials of L. Using a Combined Strategy of Metabolomics and Biological Activity Analyses.

机构信息

Corporación Colombiana de Investigación Agropecuaria-Agrosavia, Centro de Investigación La Selva, Kilómetro 7, Vía a Las Palmas, Vereda Llanogrande, Rionegro 054048, Colombia.

Grupo de Investigación en Sustancias Bioactivas GISB, Facultad de Ciencias Farmacéuticas y Alimentarias, Universidad de Antioquia, Cl. 70 No. 52-21, Medellin 0500100, Colombia.

出版信息

Molecules. 2022 May 31;27(11):3559. doi: 10.3390/molecules27113559.

DOI:10.3390/molecules27113559

PMID:35684496

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9182276/

Abstract

Spearmint ( L.) has been widely studied for its diversity of compounds for product generation. However, studies describing the chemical and biological characteristics of commercial spearmint materials from different origins are scarce. For this reason, this research aimed to bioprospecting spearmint from three origins: Colombia (Col), Mexico (Mex), and Egypt (Eg). We performed a biological activity analysis, such as FRAP, DPPH, and ABTS, inhibition potential of , , , , , Methicillin-Resistant, and Furthermore, we performed chemical assays, such as total polyphenol and rosmarinic acid, and untargeted metabolomics via HPLC-MS/MS. Finally, we developed a causality analysis to integrate biological activities with chemical analyses. We found significant differences between the samples for the total polyphenol and rosmarinic acid contents, FRAP, and inhibition analyses for Methicillin-Resistant and . Also, clear metabolic differentiation was observed among the three commercial materials evaluated. These results allow us to propose data-driven uses for the three spearmint materials available in current markets.

摘要

留兰香因其化合物的多样性而被广泛研究，可用于产品生成。然而，描述来自不同来源的商业留兰香材料的化学和生物学特性的研究却很少。基于此，本研究旨在从三个产地（哥伦比亚、墨西哥和埃及）中对留兰香进行生物勘探。我们进行了生物活性分析，如 FRAP、DPPH 和 ABTS 自由基清除能力、对和的抑制潜力、耐甲氧西林金黄色葡萄球菌和。此外，我们还进行了总多酚和迷迭香酸的化学分析以及通过 HPLC-MS/MS 的非靶向代谢组学分析。最后，我们开发了一种因果分析方法，将生物活性与化学分析相结合。我们发现样品之间在总多酚和迷迭香酸含量、FRAP 以及对耐甲氧西林金黄色葡萄球菌和的抑制分析方面存在显著差异。此外，还观察到三种商业材料之间存在明显的代谢分化。这些结果使我们能够提出对当前市场上三种留兰香材料的基于数据的用途。