Yunnan Yunzhong Research Institute of Nutrition and Health, Yunnan University of Chinese Medicine, Kunming 650500, China.
Department of Chemistry, Abbottabad Campus, COMSATS University Islamabad, Abbottabad 22060, Pakistan.
Molecules. 2022 Jun 3;27(11):3602. doi: 10.3390/molecules27113602.
Traditional Chinese Medicine is generally used as a decoction to guard health. Many active ingredients in the decoction are chemical ingredients that are not usually paid attention to in phytochemical research, such as polysaccharides, etc. Based on research interest in Chinese herbal decoction, crude polysaccharides from (GCP) were purified to obtain two relatively homogeneous polysaccharides, a neutral polysaccharide (GNP), and an acid polysaccharide (GAP) by various chromatographic separation methods, which were initially characterized by GC-MS, NMR, IR, and methylation analysis. Studies on the hepatoprotective activity of GCP in vivo showed that GCP might be a potential agent for the prevention and treatment of acute liver injury by inhibiting the secretion levels of ALT, AST, IL-6, IL-1, TNF-, and MDA expression levels, increasing SOD, and the GSH-Px activity value. Further, in vitro assays, GNP and GAP, decrease the inflammatory response by inhibiting the secretion of IL-6 and TNF-, involved in the STAT1/T-bet signaling pathway.
中药一般作为汤剂来养生。许多汤剂中的活性成分都是植物化学研究中通常不关注的化学成分，如多糖等。基于对中药汤剂的研究兴趣，从（GCP）中提取粗多糖，通过各种色谱分离方法得到两种相对均一的多糖，中性多糖（GNP）和酸性多糖（GAP），初步通过 GC-MS、NMR、IR 和甲基化分析进行了表征。体内 GCP 对肝保护活性的研究表明，GCP 可能是通过抑制 ALT、AST、IL-6、IL-1、TNF-和 MDA 表达水平的分泌，增加 SOD 和 GSH-Px 活性值来预防和治疗急性肝损伤的潜在药物。此外，体外实验表明，GNP 和 GAP 通过抑制 IL-6 和 TNF-的分泌来减轻炎症反应，涉及 STAT1/T-bet 信号通路。
