Engineering Research Center of Bio-Process of Ministry of Education, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China; School of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
Engineering Research Center of Bio-Process of Ministry of Education, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China; School of Food and Biological Engineering, Hefei University of Technology, 193 Tunxi Road, Hefei, Anhui 230009, PR China.
Carbohydr Polym. 2019 May 15;212:89-101. doi: 10.1016/j.carbpol.2019.02.031. Epub 2019 Feb 12.
Two polysaccharide fractions (TSP-1 and TSP-2) with molecular weights of 833.6 kDa and 81.6 kDa were isolated from Toona sinensis leaves (Meliaceae) by hot water extraction, DEAE Cellulose-52 chromatography and Sephacryl S-400 gel permeation chromatography. Structural analysis indicated that TSP-1 and TSP-2 consisted of Manp, GlcpA, Glcp, Galp, Xylp and Araf with different molar ratios. Methylation and NMR analysis revealed that the backbone of TSP-1 might consist of 1,6-linked-Glcp, 1,3,6-linked-Manp and 1,6-linked-Galp, while TSP-2 was mainly composed of 1,3,5-linked-Araf, 1,6-linked-Glcp, 1,4-linked-Xylp and 1,6-linked-Galp. Congo red assay indicated that TSP-1 and TSP-2 had no triple-helix structure, which was consistent with the results of AFM. In vivo hepatoprotective activity showed that TSP-1 and TSP-2 could improve CCl-induced mice liver injury by reducing the activities of AST, ALT and the level of MDA, increasing the activities of SOD, GSH-Px, and CAT and the level of GSH in liver and decreasing the expression levels of TNF-α and IL-6 in liver. These results suggest that TSP-1 and TSP-2 have promising potential to serve as hepatoprotective agents.
从香椿叶（楝科）中热水提取、DEAE 纤维素-52 层析和 Sephacryl S-400 凝胶渗透层析分离得到两种多糖级分（TSP-1 和 TSP-2），分子量分别为 833.6 kDa 和 81.6 kDa。结构分析表明，TSP-1 和 TSP-2 由 Manp、GlcpA、Glcp、Galp、Xylp 和 Araf 组成，摩尔比不同。甲基化和 NMR 分析表明，TSP-1 的骨架可能由 1,6-连接的 Glcp、1,3,6-连接的 Manp 和 1,6-连接的 Galp 组成，而 TSP-2 主要由 1,3,5-连接的 Araf、1,6-连接的 Glcp、1,4-连接的 Xylp 和 1,6-连接的 Galp 组成。刚果红试验表明，TSP-1 和 TSP-2 没有三螺旋结构，这与 AFM 的结果一致。体内保肝活性表明，TSP-1 和 TSP-2 可通过降低 AST、ALT 活性和 MDA 水平，增加 SOD、GSH-Px 和 CAT 活性以及 GSH 水平，降低 TNF-α和 IL-6 在肝脏中的表达水平，改善 CCl 诱导的小鼠肝损伤。这些结果表明，TSP-1 和 TSP-2 具有作为保肝剂的潜在应用前景。
