Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore 641 003, India.
Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641 003, India.
Molecules. 2022 Jun 6;27(11):3631. doi: 10.3390/molecules27113631.
In this study, the volatilomes of naturally growing plant leaves were immobilized in a suitable substrate to enhance vapors' diffusion in the soil to eradicate the wilt pathogens in Tomato. Volatilomes produced by leaves immobilized in vermiculite ball was found to be effective and exhibit 92.35 percent inhibition on the mycelial growth of f. sp. (FOL). Moreover, the volatilomes of immobilized vermiculite balls were tested based on the distance traveled by the diffused volatilomes from the ball and revealed that the volatilomes of traveled up to 20 cm distance from the center of PVC (Polyvinly chloride) chamber showed maximum reduction in colony growth of FOL at 12th day after inoculation. Tomato plants inoculated with FOL revealed increased expressions of defense gene, pathogenesis related protein (PR1) with 2.63-fold after 72 h and the gene, transcription factor (WRKY) increased with 2.5-fold after 48 h on exposure to the volatilomes of vermiculite balls. To the best of our knowledge, this is the first report on development of volatilomes based vermiculite ball formulations. This result indicated that the volatilomes of are promising phyto-fumigants for management of Tomato wilt.
在这项研究中，将自然生长的植物叶片的挥发物固定在合适的基质中，以增强挥发物在土壤中的扩散，从而根除番茄枯萎病的病原体。研究发现，固定在蛭石球中的叶片挥发物具有很好的效果，对 F. sp.（FOL）的菌丝生长抑制率达到 92.35%。此外，还基于从球中扩散的挥发物的行进距离对固定化蛭石球的挥发物进行了测试，结果表明，从 PVC（聚氯乙烯）室中心扩散出的挥发物在 12 天的接种后，在 20 厘米的距离内，对 FOL 的菌落生长有最大的抑制作用。接种 FOL 的番茄植株在暴露于蛭石球的挥发物 72 小时后，防御基因、病程相关蛋白（PR1）的表达增加了 2.63 倍，转录因子（WRKY）的基因在 48 小时后增加了 2.5 倍。据我们所知，这是首次报道基于挥发物的蛭石球制剂的开发。该结果表明，的挥发物有望成为防治番茄枯萎病的植物熏蒸剂。
