Department of Biological Sciences, Virginia Tech, Blacksburg, VA, 24061, USA.
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC, 27695, USA.
Plant J. 2021 Apr;106(2):314-325. doi: 10.1111/tpj.15176. Epub 2021 Apr 5.
Volatile organic compounds (VOCs) released by plants serve as information and defense chemicals in mutualistic and antagonistic interactions and mitigate effects of abiotic stress. Passive and dynamic sampling techniques combined with gas chromatography-mass spectrometry analysis have become routine tools to measure emissions of VOCs and determine their various functions. More recently, knowledge of the roles of plant VOCs in the aboveground environment has led to the exploration of similar functions in the soil and rhizosphere. Moreover, VOC patterns have been recognized as sensitive and time-dependent markers of biotic and abiotic stress. This focused review addresses these developments by presenting recent progress in VOC sampling and analysis. We show advances in the use of small, inexpensive sampling devices and describe methods to monitor plant VOC emissions in the belowground environment. We further address latest trends in real-time measurements of volatilomes in plant phenotyping and most recent developments of small portable devices and VOC sensors for non-invasive VOC fingerprinting of plant disease. These technologies allow for innovative approaches to study plant VOC biology and application in agriculture.
植物释放的挥发性有机化合物（VOCs）在互利和拮抗相互作用中充当信息和防御化学物质，并减轻非生物胁迫的影响。被动和动态采样技术与气相色谱-质谱分析相结合，已成为测量 VOC 排放并确定其各种功能的常规工具。最近，人们对植物 VOC 在地上环境中的作用的认识导致了对土壤和根际中类似功能的探索。此外，VOC 模式已被认为是生物和非生物胁迫的敏感和时变标志物。本重点综述通过介绍 VOC 采样和分析的最新进展来阐述这些发展。我们展示了使用小型、廉价采样设备的进展，并描述了监测地下环境中植物 VOC 排放的方法。我们进一步探讨了植物表型实时测量挥发物组学的最新趋势，以及小型便携式设备和 VOC 传感器的最新发展，用于植物疾病的非侵入性 VOC 指纹识别。这些技术为研究植物 VOC 生物学及其在农业中的应用提供了创新方法。
