Liu Jiming, Gao Shilun, Xu Yuanyuan, Wang Mianzhi, Ngiam Jia Jun, Rui Wen Nicholas Cho, Yi Joan Jong Jing, Weng Xuehuang, Jia Liming, Salojärvi Jarkko
Key Laboratory for Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing, China.
National Energy R&D Center for Non-Food Biamass, Beijing Forestry University, Beijing, China.
Front Plant Sci. 2022 May 24;13:857993. doi: 10.3389/fpls.2022.857993. eCollection 2022.
is an important forest tree genus with utilization in biodiesel, biomedicine, and it harbors great potential for biochemical engineering applications. For advanced breeding of , it is necessary to evaluate the genetic diversity and construct a rationally designed core germplasm collection. In this study, the genetic diversity and population structure of were conducted with 18 expressed sequence tag-simple sequence repeat (EST-SSR) markers in order to establish a core germplasm collection from 161 accessions. The population of showed high genetic diversity and significant population structure. Interspecific genetic variation was significantly higher than intraspecific variation in the , , and combined plus var. populations. had abundant genetic variation and showed a specific pattern of geographical variation, whereas , , and var. showed less intraspecific variation. A core germplasm collection was created that contained 40% of genetic variation in the initial population, comprising 53 and nine lineages, as well as single representatives of and var. These results provide a germplasm basis and theoretical rationale for the efficient management, conservation, and utilization of , as well as genetic resources for joint genomics research in the future.
是一个重要的林木属,在生物柴油、生物医药领域有应用,并且在生化工程应用方面具有巨大潜力。对于的高级育种,有必要评估遗传多样性并构建合理设计的核心种质库。在本研究中,利用18个表达序列标签-简单序列重复(EST-SSR)标记对的遗传多样性和群体结构进行了分析,以便从161份种质中建立核心种质库。的群体表现出高遗传多样性和显著的群体结构。在、和的群体以及和变种的混合群体中,种间遗传变异显著高于种内变异。具有丰富的遗传变异并呈现出特定的地理变异模式,而、和变种的种内变异较少。创建了一个核心种质库,其包含初始群体40%的遗传变异,由53份种质和9个谱系组成,以及和变种的单份代表。这些结果为的有效管理、保护和利用提供了种质基础和理论依据,也为未来联合基因组学研究提供了遗传资源。