Ren Deqiang, Wu Wenwen, Wen Qinqin, Li Yujiao
College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, China.
PeerJ. 2025 Jun 17;13:e19560. doi: 10.7717/peerj.19560. eCollection 2025.
is a commonly used medicine for the Miao nationality distributed over Guizhou and used to treat laryngeal diseases. The medicinal materials of the come from various sources, including Bailiangjin ( (Thunb.) A. DC.), Zhushagen ( Sims), Hongliangsan ( Sims var. bicolor (Walker) C. Y. Wu et C. Chen), Xibingbailiangjin ( (Thunb.) A. DC. var. dielsii (Levl.) Walker) and Dayebailiangjin ( (Thunb.) A. DC. var. amplifolia Walker). With the continuous improvement of the medicinal value of the Miao medicine and the increasing scarcity of wild resources, it is of great practical significance to solve the problems of effective identification and genetic structure analysis of medicinal materials and adulterants, for the protection of its germplasm resources and the protection of clinical drug safety. The development of expressed sequence tag-simple sequence repeat (EST-SSR) based on transcriptome strategy is considered to be a very effective means. In this study, 51,237 sequences of were retrieved, with a total length of 71.4 MB. A total of 32,827 SSR loci were detected, averaging one SSR locus per 2.1 KB. The distribution of primers was detected, and 28,322 SSR loci were unigene SSR. 32,827 pairs of EST-SSR molecular markers were developed for the whole genome, with an average of 0.64 pairs of SSR primers for each unigene, and the sequence coverage was high. The statistical analysis showed that six types of SSR nucleotides could be detected, but the number and frequency of EST-SSR in different primitive types were significantly different. Mononucleotide and dinucleotide were the main repeat types, accounting for 90.51% of the total SSR of . Sixty pairs of primers were randomly selected and applied to genetic research. Among them, 51 pairs could amplify 200 polymorphic bands. Genetic analysis was carried out on 46 mixed species of . The results showed that the original plants of showed high genetic diversity and could be divided into two populations. The results of systematic clustering showed that the EST-SSR used could well distinguish and its easily mixed species; it can be applied to the identification of , as well as the molecular identification between the original Bailiangjin, Zhushagen and Hongliangsan. This study can provide a reference for the genetic analysis of the .
是一种分布于贵州的苗族常用药，用于治疗咽喉疾病。该药材的药用材料来源多样，包括白凉金（(Thunb.) A. DC.）、朱砂根（Sims）、红凉伞（Sims var. bicolor (Walker) C. Y. Wu et C. Chen）、细柄白凉金（(Thunb.) A. DC. var. dielsii (Levl.) Walker）和大叶白凉金（(Thunb.) A. DC. var. amplifolia Walker）。随着苗药药用价值的不断提高以及野生资源的日益稀缺，解决药材及其掺伪品的有效鉴定和遗传结构分析问题，对于保护其种质资源和保障临床用药安全具有重要的现实意义。基于转录组策略开发表达序列标签 - 简单序列重复（EST - SSR）被认为是一种非常有效的手段。本研究共检索到该药材的51237条序列，总长71.4 MB。共检测到32827个SSR位点，平均每2.1 KB有一个SSR位点。检测了引物的分布情况，其中28322个SSR位点为单基因SSR。针对全基因组开发了32827对EST - SSR分子标记，平均每个单基因有0.64对SSR引物，序列覆盖率高。统计分析表明可检测到六种类型的SSR核苷酸，但不同原始类型中EST - SSR的数量和频率存在显著差异。单核苷酸和二核苷酸是主要的重复类型，占该药材总SSR的90.51%。随机选取60对引物应用于遗传研究。其中51对能扩增出200条多态性条带。对该药材的46个混伪种进行了遗传分析。结果表明该药材的原植物显示出较高的遗传多样性，可分为两个种群。系统聚类结果表明，所使用的EST - SSR能够很好地区分该药材及其易混淆物种；可应用于该药材的鉴定，以及原植物白凉金、朱砂根和红凉伞之间的分子鉴定。本研究可为该药材的遗传分析提供参考。
