Navarro Alvaro M, Orti Fernando, Martínez-Pérez Elizabeth, Alonso Macarena, Simonetti Franco L, Iserte Javier A, Marino-Buslje Cristina
Fundación Instituto Leloir. Av. Patricias Argentinas 435, C1405BWE Buenos Aires, Argentina.
Comput Struct Biotechnol J. 2022 May 12;20:2551-2557. doi: 10.1016/j.csbj.2022.05.004. eCollection 2022.
Proteins involved in liquid-liquid phase separation (LLPS) and membraneless organelles (MLOs) are recognized to be decisive for many biological processes and also responsible for several diseases. The recent explosion of research in the area still lacks tools for the analysis and data integration among different repositories. Currently, there is not a comprehensive and dedicated database that collects all disease-related variations in combination with the protein location, biological role in the MLO, and all the metadata available for each protein and disease. Disease-related protein variants and additional features are dispersed and the user has to navigate many databases, with a different focus, formats, and often not user friendly.
We present DisPhaseDB, a database dedicated to disease-related variants of liquid-liquid phase separation proteins. It integrates 10 databases, contains 5,741 proteins, 1,660,059 variants, and 4,051 disease terms. It also offers intuitive navigation and an informative display. It constitutes a pivotal starting point for further analysis, encouraging the development of new computational tools.The database is freely available at http://disphasedb.leloir.org.ar.
参与液-液相分离（LLPS）和无膜细胞器（MLO）的蛋白质被认为对许多生物过程起决定性作用，并且也是多种疾病的病因。该领域最近的研究激增，但仍缺乏用于不同数据库之间分析和数据整合的工具。目前，尚无一个全面且专门的数据库来收集所有与疾病相关的变异，并结合蛋白质定位、在MLO中的生物学作用以及每种蛋白质和疾病的所有可用元数据。与疾病相关的蛋白质变体和其他特征分散在各处，用户必须浏览许多数据库，这些数据库重点不同、格式各异，而且通常对用户不友好。
我们展示了DisPhaseDB，这是一个专门针对液-液相分离蛋白质的疾病相关变体的数据库。它整合了10个数据库，包含5741种蛋白质、1660059个变体和4051个疾病术语。它还提供直观的导航和信息丰富的展示。它构成了进一步分析的关键起点，有助于推动新计算工具的开发。该数据库可在http://disphasedb.leloir.org.ar免费获取。
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