Chen 陈韬宇 Taoyu, Tang 唐果菓 Guoguo, Li 李天昊 Tianhao, Yanghong 杨宏芷宁 Zhining, Hou 侯超 Chao, Du 杜泽州 Zezhou, You 游铠强 Kaiqiang, Ma 马利伟 Liwei, Li 李婷婷 Tingting
Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Key Laboratory for Neuroscience, Ministry of Education / National Health Commission of China, Peking University, Beijing 100191, China.
Genomics Proteomics Bioinformatics. 2025 May 10;23(1). doi: 10.1093/gpbjnl/qzaf014.
Biomacromolecules form membraneless organelles through liquid-liquid phase separation in order to regulate the efficiency of particular biochemical reactions. Dysregulation of phase separation might result in pathological condensation or sequestration of biomolecules, leading to diseases. Thus, phase separation and phase separating factors may serve as drug targets for disease treatment. Nevertheless, such associations have not yet been integrated into phase separation-related databases. Therefore, based on MloDisDB, a database for membraneless organelle factor-disease associations previously developed by our lab, we constructed PhaSeDis, the phase separation-disease association database. We increased the number of phase separation entries from 52 to 185, and supplemented the evidence provided by the original articles verifying the phase separation nature of the factors. Moreover, we included the information of interacting small molecules with low-throughput or high-throughput evidence that might serve as potential drugs for phase separation entries. PhaSeDis strives to offer comprehensive descriptions of each entry, elucidating how phase separating factors induce pathological conditions via phase separation and the mechanisms by which small molecules intervene. We believe that PhaSeDis would be very important in the application of phase separation regulation in treating related diseases. PhaSeDis is available at http://mlodis.phasep.pro.
生物大分子通过液-液相分离形成无膜细胞器，以调节特定生化反应的效率。相分离失调可能导致生物分子的病理性凝聚或隔离，从而引发疾病。因此，相分离和相分离因子可作为疾病治疗的药物靶点。然而，此类关联尚未整合到与相分离相关的数据库中。因此，基于我们实验室之前开发的无膜细胞器因子-疾病关联数据库MloDisDB，我们构建了相分离-疾病关联数据库PhaSeDis。我们将相分离条目的数量从52个增加到185个，并补充了原始文章提供的验证因子相分离性质的证据。此外，我们纳入了具有低通量或高通量证据的与相分离条目相互作用的小分子信息，这些小分子可能作为潜在药物。PhaSeDis致力于对每个条目进行全面描述，阐明相分离因子如何通过相分离诱导病理状态以及小分子干预的机制。我们相信PhaSeDis在相分离调控治疗相关疾病的应用中将非常重要。PhaSeDis可在http://mlodis.phasep.pro获取。
