TaqMan Probe-Based Quantitative Real-Time PCR to Detect in Traditional Chinese Patent Medicines.

There has been global concern about the safety and accuracy of traditional Chinese patent medicines (TCPMs). , also known as sanqi, is an important constituent of TCPMs. However, identifying the species contained in TCPMs is challenging due to the presence of multiple ingredients and the use of various preparation processes. To detect in TCPMs. A TaqMan probe-based qPCR assay was constructed and validated with DNA extracted from and adulterants. In total, 75 samples derived from 25 batches of TCPMs were tested using the constructed qPCR method. A TaqMan probe-based qPCR assay targeting was established. The constructed qPCR assay could specifically discriminate from , and Salisb. cv. Wenyujin. The sensitivity study showed that the detectable DNA template concentration of for this qPCR assay was 0.001 ng/μl. All 75 samples from TCPMs were confirmed to contain by the qPCR assay. The qPCR method can accurately identify in TCPMs and is promising as a powerful tool for quality control and market regulation.