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用不同黏液/胶体制备的藻酸盐珠对产罗伊氏菌素菌进行有效包封。

Effective encapsulation of reuterin-producing in alginate beads prepared with different mucilages/gums.

作者信息

Rodrigues F J, Cedran M F, Pereira G A, Bicas J L, Sato H H

机构信息

Food Biochemistry Laboratory, Department of Food Science and Nutrition, School of Food Engineering, University of Campinas, Campinas, SP, Brazil.

Food Biotechnology Laboratory, Department of Food Science and Nutrition, School of Food Engineering, University of Campinas, Campinas, SP, Brazil.

出版信息

Biotechnol Rep (Amst). 2022 May 14;34:e00737. doi: 10.1016/j.btre.2022.e00737. eCollection 2022 Jun.

Abstract

The mainly aim of this study was to use mucilaginous solutions obtained from tamarind, mutamba, cassia tora, psyllium and konjac powdered to encapsulate reuterin-producing in alginate beads by extrusion technique. In the particles were determined the bacterial encapsulation efficiency, cell viability during storage and survival under simulated gastric and intestinal conditions. Moreover, the reuterin production, its entrapment into the beads and the influence on viability of encapsulated microorganism were evaluated. Scanning electron microscopy and Fourier Transform Infrared spectroscopy were employed to characterize the produced particles. The beads showed a relatively spherical shape with homogenous distribution of . The use of gums and mucilages combined with alginate improved the encapsulation efficiency (from 93.2 to 97.4%), the viability of encapsulated bacteria during refrigerated storage (especially in prolonged storage of 20, 30 and 60 days) and the survival after exposure to gastric and enteric environments (from 67.7 to 76.6%). The was able to produce reuterin via bioconversion of glycerol in the film-forming solutions, and the entrapment of the metabolite was improved using konjac, mutamba and tamarind mucilaginous solutions in the encapsulation process (45, 44.57 and 41.25%, respectively). Thus, our findings confirm the great potential of these hydrocolloids to different further purposes, enabling its application as support material for delivery of chemical or biological compounds.

摘要

本研究的主要目的是使用从罗望子、穆坦巴、决明子、车前草和魔芋粉中获得的黏液溶液,通过挤压技术将产生罗伊氏菌素的菌封装在海藻酸钠珠中。测定了颗粒中的细菌封装效率、储存期间的细胞活力以及在模拟胃和肠道条件下的存活率。此外,还评估了罗伊氏菌素的产生、其在珠中的包封以及对封装微生物活力的影响。采用扫描电子显微镜和傅里叶变换红外光谱对所制备的颗粒进行表征。这些珠子呈现出相对球形的形状,且分布均匀。使用树胶和黏液与海藻酸钠相结合提高了封装效率(从93.2%提高到97.4%)、冷藏储存期间封装细菌的活力(特别是在20、30和60天的长期储存中)以及暴露于胃和肠道环境后的存活率(从67.7%提高到76.6%)。该菌能够通过在成膜溶液中对甘油进行生物转化来产生罗伊氏菌素,并且在封装过程中使用魔芋、穆坦巴和罗望子黏液溶液提高了代谢产物的包封率(分别为45%、44.57%和41.25%)。因此,我们的研究结果证实了这些水胶体在不同进一步用途方面具有巨大潜力,使其能够作为化学或生物化合物递送的支撑材料应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/096d/9171447/7218b3240465/ga1.jpg

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