Cadieux Peter, Wind Anette, Sommer Philip, Schaefer Laura, Crowley Kate, Britton Robert A, Reid Gregor
Lawson Health Research Institute, University of Western Ontario, 268 Grosvenor Street, London, Ontario N6A 4V2, Canada.
Appl Environ Microbiol. 2008 Aug;74(15):4645-9. doi: 10.1128/AEM.00139-08. Epub 2008 Jun 6.
Classified as a distinct species in 1980, Lactobacillus reuteri strains have been used in probiotic formulations for intestinal and urogenital applications. In the former, the primary mechanism of action of L. reuteri SD2112 (ATCC 55730) has been purported to be its ability to produce the antibiotic 3-hydroxypropionaldehyde (3-HPA), also known as reuterin. In the vagina, it has been postulated that probiotic Lactobacillus reuteri RC-14 does not require reuterin production but mediates a restoration of the normal microbiota via hydrogen peroxide, biosurfactant, lactic acid production, and immune modulation. The aim of the present study was to determine whether strain RC-14 produced reuterin. Using PCR and DNA dot blot analyses, numerous Lactobacillus species, including RC-14, were screened for the presence of the gene encoding the large subunit of glycerol dehydratase (gldC), the enzyme responsible for reuterin production. In addition, lactobacilli were grown in glycerol-based media and both high-performance liquid chromatography and a colorimetric assay were used to test for the presence of reuterin. L. reuteri RC-14 was determined to be negative for gldC sequences, as well as for the production of reuterin when cultured in the presence of glycerol. These findings support that the probiotic effects of L. reuteri RC-14, repeatedly demonstrated during numerous studies of the intestine and vagina, are independent of reuterin production.
罗伊氏乳杆菌菌株于1980年被分类为一个独特的物种，已被用于肠道和泌尿生殖系统应用的益生菌制剂中。在前者中，罗伊氏乳杆菌SD2112（ATCC 55730）的主要作用机制据说是其产生抗生素3-羟基丙醛（3-HPA，也称为罗伊菌素）的能力。在阴道中，据推测，益生菌罗伊氏乳杆菌RC-14不需要产生罗伊菌素，而是通过过氧化氢、生物表面活性剂、乳酸产生和免疫调节来介导正常微生物群的恢复。本研究的目的是确定RC-14菌株是否产生罗伊菌素。使用聚合酶链反应（PCR）和DNA斑点印迹分析，对包括RC-14在内的众多乳杆菌物种进行筛选，以检测编码甘油脱水酶大亚基（gldC）的基因的存在，该酶负责罗伊菌素的产生。此外，将乳杆菌在基于甘油的培养基中培养，并使用高效液相色谱法和比色法检测罗伊菌素的存在。罗伊氏乳杆菌RC-14被确定为gldC序列阴性，并且在甘油存在下培养时也不产生罗伊菌素。这些发现支持了罗伊氏乳杆菌RC-14的益生菌作用，在对肠道和阴道的众多研究中反复证明，其与罗伊菌素的产生无关。
