Department of Mechanical Engineering Korea Advanced Institute of Science and Technology Daejeon 34141 Republic of Korea.
School of Mechanical Engineering Chonnam National University Gwangju 61186 Republic of Korea.
Adv Sci (Weinh). 2022 Mar 25;9(16):2105809. doi: 10.1002/advs.202105809. eCollection 2022 Jun.
The cytotoxic response of natural killer (NK) cells in a microreactor to surface acoustic waves (SAWs) is investigated, where the SAWs produce an acoustic streaming flow. The Rayleigh-type SAWs form by an interdigital transducer propagated along the surface of a piezoelectric substrate in order to allow the dynamic stimulation of functional immune cells in a noncontact and rotor-free manner. The developed acoustofluidic microreactor enables a dynamic cell culture to be set up in a miniaturized system while maintaining the performance of agitating media. The present SAW system creates acoustic streaming flow in the cylindrical microreactor and applies flow-induced shear stress to the cells. The suspended NK cells are found to not be damaged by the SAW operation of the adjusted experimental setup. Suspended NK cell aggregates subjected to an SAW treatment show increased intracellular Ca concentrations. Simultaneously treating the NK cells with SAWs and protein kinase C activator enhances the lysosomal protein expressions of the cells and the cell-mediated cytotoxicity against target tumor cells. These have important implications by showing that acoustically actuated system allows dynamic cell culture without cell damages and further alters cytotoxicity-related cellular activities.
研究了在微反应器中，表面声波（SAW）对自然杀伤（NK）细胞的细胞毒性反应，其中 SAW 产生声流。瑞利型 SAW 通过沿压电衬底表面传播的叉指换能器形成，以便能够以非接触和无转子的方式动态刺激功能免疫细胞。所开发的声流微反应器能够在保持搅拌介质性能的同时，在小型化系统中建立动态细胞培养。目前的 SAW 系统在圆柱形微反应器中产生声流，并向细胞施加流致剪切应力。发现悬浮的 NK 细胞不会因调整后的实验设置的 SAW 操作而受损。悬浮的 NK 细胞聚集体在接受 SAW 处理后，细胞内 Ca 浓度增加。同时用 SAW 和蛋白激酶 C 激活剂处理 NK 细胞，增强了细胞的溶酶体蛋白表达和细胞对靶肿瘤细胞的细胞介导的细胞毒性。这些结果表明，声激活系统允许在不损伤细胞的情况下进行动态细胞培养，并进一步改变与细胞毒性相关的细胞活性，这具有重要意义。
