Walker Logan, Subramaniam Kuttichantran, Viadanna Pedro H O, Vann Jordan A, Marcquenski Susan, Godard Danielle, Kieran Elizabeth, Frasca Salvatore, Popov Vsevolod L, Kerr Karen, Davison Andrew J, Waltzek Thomas B
Fisheries and Aquatic Sciences, College of Agricultural and Life Sciences, University of Florida, Gainesville, FL 32611, USA.
Dis Aquat Organ. 2022 Jun 2;149:83-96. doi: 10.3354/dao03661.
In the spring of 2017, 2 adult lake sturgeon (LS) Acipenser fulvescens captured from the Wolf River, Wisconsin (USA), presented with multiple cutaneous plaques that, upon microscopic examination, indicated proliferative epidermitis. Ultrastructural examination of affected keratinocytes revealed particles in the nucleus having a morphology typical of herpesviruses. A degenerate PCR assay targeting the DNA polymerase catalytic subunit (pol) gene of large double-stranded DNA viruses generated amplicons of the anticipated size from skin samples, and sequences of amplicons confirmed the presence of a novel alloherpesvirus (lake sturgeon herpesvirus, LSHV) related to acipenserid herpesvirus 1 (AciHV1). The complete genome (202660 bp) of this virus was sequenced using a MiSeq System, and phylogenetic analyses substantiated the close relationship to AciHV1. A PCR assay targeting the LSHV DNA packaging terminase subunit 1 (ter1) gene demonstrated the presence of the virus in 39/42 skin lesion samples collected from wild LS captured in 2017-2019 and 2021 in 4/4 rivers in Wisconsin. Future efforts to isolate LSHV in cell culture would facilitate challenge studies to determine the disease potential of the virus.
2017年春季，从美国威斯康星州沃尔夫河捕获的2条成年湖鲟（Acipenser fulvescens）出现多处皮肤斑块，经显微镜检查显示为增殖性表皮炎。对受影响的角质形成细胞进行超微结构检查，发现细胞核内有形态典型的疱疹病毒颗粒。针对大型双链DNA病毒的DNA聚合酶催化亚基（pol）基因进行的简并PCR检测，从皮肤样本中扩增出预期大小的片段，扩增片段序列证实存在一种与鲟鱼疱疹病毒1型（AciHV1）相关的新型异疱疹病毒（湖鲟疱疹病毒，LSHV）。使用MiSeq系统对该病毒的完整基因组（202660 bp）进行了测序，系统发育分析证实了其与AciHV1的密切关系。针对LSHV DNA包装末端酶亚基1（ter1）基因的PCR检测表明，在2017 - 2019年和2021年从威斯康星州4条河流捕获的野生湖鲟采集的42份皮肤病变样本中，有39份检测到该病毒。未来在细胞培养中分离LSHV的工作将有助于开展攻毒研究，以确定该病毒的致病潜力。
