Garver Kyle A, Leskisenoja Katja, Macrae Robert, Hawley Laura M, Subramaniam Kuttichantran, Waltzek Thomas B, Richard Jon, Josefsson Caroline, Valtonen E Tellervo
Fisheries and Oceans Canada, Pacific Biological Station, Nanaimo, British Columbia V9T 6N7, Canada.
Dis Aquat Organ. 2018 Jun 4;128(3):175-185. doi: 10.3354/dao03228.
The order Herpesvirales includes viruses that infect aquatic and terrestrial vertebrates and several aquatic invertebrates (i.e. mollusks), and share the commonality of possessing a double-stranded DNA core surrounded by an icosahedral capsid. Herpesviruses of the family Alloherpesviridae that infect fish and amphibians, including channel catfish virus and koi herpesvirus, negatively impact aquaculture. Here, we describe a novel herpesvirus infection of wild European perch from lakes in Finland. Infected fish exhibited white nodules on the skin and fins, typically in the spring when prevalence reached nearly 40% in one of the sampled lakes. Transmission electron microscopic examination of affected tissues revealed abundant nuclear and cytoplasmic virus particles displaying herpesvirus morphology. Degenerate PCR targeting a conserved region of the DNA polymerase gene of large DNA viruses amplified a 520 bp product in 5 of 5 affected perch skin samples tested. Phylogenetic analysis of concatenated partial DNA polymerase and terminase (exon 2) gene sequences produced a well-supported tree grouping the European perch herpesvirus with alloherpesviruses infecting acipenserid, esocid, ictalurid, and salmonid fishes. The phenetic analysis of the European perch herpesvirus partial DNA polymerase and terminase nucleotide gene sequences ranged from 34.6 to 63.9% and 39.6 to 59.6% to other alloherpesviruses, respectively. These data support the European perch herpesvirus as a new alloherpesvirus, and we propose the formal species designation of Percid herpesvirus 2 (PeHV2) to be considered for approval by the International Committee on Taxonomy of Viruses.
疱疹病毒目包括感染水生和陆生脊椎动物以及几种水生无脊椎动物（即软体动物）的病毒，它们的共同特点是拥有一个被二十面体衣壳包围的双链DNA核心。感染鱼类和两栖动物的异疱疹病毒科疱疹病毒，包括斑点叉尾鮰病毒和锦鲤疱疹病毒，对水产养殖产生负面影响。在此，我们描述了一种来自芬兰湖泊野生欧洲鲈的新型疱疹病毒感染。受感染的鱼在皮肤和鳍上出现白色结节，通常在春季，其中一个采样湖泊的患病率达到近40%。对受影响组织的透射电子显微镜检查显示，大量核内和胞质内病毒颗粒呈现疱疹病毒形态。针对大型DNA病毒DNA聚合酶基因保守区域的简并PCR在5个受影响的鲈鱼皮肤样本中的5个中扩增出一个520 bp的产物。对串联的部分DNA聚合酶和末端酶（外显子2）基因序列进行系统发育分析，得到一棵支持良好的树，将欧洲鲈疱疹病毒与感染鲟科、狗鱼科、鮰科和鲑科鱼类的异疱疹病毒归为一类。欧洲鲈疱疹病毒部分DNA聚合酶和末端酶核苷酸基因序列与其他异疱疹病毒的表型分析分别为34.6%至63.9%和39.6%至59.6%。这些数据支持欧洲鲈疱疹病毒是一种新的异疱疹病毒，我们提议正式将其命名为鲈疱疹病毒2（PeHV2），以供国际病毒分类委员会批准。
