Food Functionality Research Division, Korea Food Research Institute, Jeollabuk-do 55365, Republic of Korea.
Department of Anatomy, Woosuk University, Jeollabuk-do 55338, Republic of Korea.
Food Funct. 2022 Jul 18;13(14):7494-7506. doi: 10.1039/d1fo04152e.
Radish ( L.) is a rich source of nutrients and its greens have reported functionalities. This study aimed to investigate the effects of a water-soluble extract from radish greens (WERG) on adipogenesis in 3T3-L1 adipocytes and high-fat diet-induced obesity in model mice. We also quantified the phytochemical composition of WERG such as glucoraphenin and ferulic acid. These findings show that treatment with 100 μg mL WERG reduced lipid accumulation in 3T3-L1 adipocytes, whereas in mice, the administration of 100 mg kg WERG reduced weight gain and hepatic lipid accumulation and improved the levels of serum lipid biomarkers. Furthermore, WERG treatment improved intestinal permeability and suppressed the activities of harmful intestinal enzymes in feces, thus improving gut health. It also inhibited metabolic endotoxemia and inflammatory marker levels in serum. Moreover, WERG reduced the expression of lipid-metabolism-related proteins in the liver and white adipose tissue. Collectively, these results indicate that WERG may potentiate the anti-obesity effect by improving gut health and regulating lipid metabolism.
萝卜（L.）是一种营养丰富的食物，其茎叶也具有多种功能。本研究旨在探讨萝卜茎叶水溶性提取物（WERG）对 3T3-L1 脂肪细胞脂肪生成的影响，以及对高脂肪饮食诱导肥胖模型小鼠的作用。同时，我们还定量分析了 WERG 中的植物化学物质，如葡萄糖芸苔素和阿魏酸。这些发现表明，用 100μg mL 的 WERG 处理可减少 3T3-L1 脂肪细胞中的脂质积累，而在小鼠中，用 100mg kg 的 WERG 处理可减少体重增加和肝脏脂质积累，并改善血清脂质生物标志物水平。此外，WERG 处理可改善肠道通透性并抑制粪便中有害肠道酶的活性，从而改善肠道健康。它还抑制了血清中代谢性内毒素血症和炎症标志物的水平。此外，WERG 还降低了肝脏和白色脂肪组织中与脂质代谢相关蛋白的表达。综上所述，这些结果表明，WERG 可能通过改善肠道健康和调节脂质代谢来增强其抗肥胖作用。
